Fluid-flow-induced mesenchymal stem cell migration: Role of focal adhesion kinase and RhoA kinase sensors

Brandon D. Riehl, Jeong Soon Lee, Ligyeom Ha, Jung Yul Lim

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The study of mesenchymal stem cell (MSC) migration under flow conditions with investigation of the underlying molecular mechanism could lead to a better understanding and outcome in stem-cell-based cell therapy and regenerative medicine. We used peer-reviewed open source software to develop methods for efficiently and accurately tracking, measuring and processing cell migration as well as morphology. Using these tools, we investigated MSC migration under flow-induced shear and tested the molecular mechanism with stable knockdown of focal adhesion kinase (FAK) and RhoA kinase (ROCK). Under steady flow, MSCs migrated following the flow direction in a shear stress magnitude-dependent manner, as assessed by root mean square displacement and mean square displacement, motility coefficient and confinement ratio. Silencing FAKinMSCs suppressed morphology adaptation capability and reduced cellular motility for both static and flow conditions. Interestingly, ROCK silencing significantly increased migration tendency especially under flow. Blocking ROCK, which is known to reduce cytoskeletal tension, may lower the resistance to skeletal remodelling during the flowinduced migration. Our data thus propose a potentially differential role of focal adhesion and cytoskeletal tension signalling elements in MSC migration under flow shear.

Original languageEnglish (US)
Article number20141351
JournalJournal of the Royal Society Interface
Volume12
Issue number104
DOIs
StatePublished - Mar 6 2015

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Keywords

  • Fluid shear
  • Focal adhesion kinase
  • RhoA kinase
  • Stem cell migration
  • Time lapse

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

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