Flt3 ligand augmentation of T cell mitogenesis and expansion of type 1 effector/memory T cells

R Lee Mosley, Prahlad Parajuli, Vladimir Pisarev, Jennifer Chavez, Amy Meeks, Amy Steffel, Cheryl Leutzinger, James E Talmadge

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Herein we report mechanisms whereby Flt3 ligand (FL) augments steady state T cell activity in addition to the expansion of dendritic cells (DCs). We demonstrate that in vivo administration of FL increases the frequency and absolute number of effector/memory T cells and preferentially expands T cells that express a type-1 cytokine phenotype. In addition, FL enhances T cell proliferative responses to Concanavalin A that directly correlated with increased frequencies in effector/memory T cells and expansion of lymphoid-derived (type 1) DCs (DC1s). Together, these data demonstrate that mechanisms of FL-induced T cell regulation include not only the expansion of DC subsets, but also the preferential expansion of type 1-effector/memory T cell populations, and suggest multiple mechanisms of action for FL as a vaccine adjuvant and as a therapeutic modality.

Original languageEnglish (US)
Pages (from-to)925-940
Number of pages16
JournalInternational Immunopharmacology
Volume2
Issue number7
DOIs
StatePublished - Jul 22 2002

Fingerprint

T-Lymphocytes
Dendritic Cells
Concanavalin A
flt3 ligand protein
Vaccines
Cytokines
Phenotype
Population
Therapeutics

Keywords

  • Dendritic cells
  • Flt3 ligand
  • Interferon-γ
  • Interleukin-4
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Flt3 ligand augmentation of T cell mitogenesis and expansion of type 1 effector/memory T cells. / Mosley, R Lee; Parajuli, Prahlad; Pisarev, Vladimir; Chavez, Jennifer; Meeks, Amy; Steffel, Amy; Leutzinger, Cheryl; Talmadge, James E.

In: International Immunopharmacology, Vol. 2, No. 7, 22.07.2002, p. 925-940.

Research output: Contribution to journalArticle

Mosley, R Lee ; Parajuli, Prahlad ; Pisarev, Vladimir ; Chavez, Jennifer ; Meeks, Amy ; Steffel, Amy ; Leutzinger, Cheryl ; Talmadge, James E. / Flt3 ligand augmentation of T cell mitogenesis and expansion of type 1 effector/memory T cells. In: International Immunopharmacology. 2002 ; Vol. 2, No. 7. pp. 925-940.
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