Flocculation of wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 by calcium and methanol

Matt Scholz, Takanori Hoshino, Daniel Johnson, Mark Riley, Joel Cuello

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 ± 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 ± 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered that the addition of small amounts (<5% v/v) of methanol could significantly reduce the calcium needed to achieve flocculation. Within 15 min after addition of 12 mM calcium chloride and 4.6% (v/v) methanol, 83 ± 4% of cw15 cells flocculated. Methanol is fully recoverable by distillation, and its use might enable flocculation without further water salinization when media calcium concentrations fall short of 15 mM. It was further shown that substrates for and/or products of cellular growth affected flocculation adversely. Nearly 81% of cells flocculated from fresh medium compared to only 54% in spent medium.

Original languageEnglish (US)
Pages (from-to)4835-4840
Number of pages6
JournalBiomass and Bioenergy
Volume35
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

flocculation
Chlamydomonas reinhardtii
Flocculation
algae
methanol
Calcium
Methanol
calcium
mutants
Calcium chloride
Algae
calcium chloride
cells
water salinization
alga
chloride
distillation
Distillation
starvation
wastewater

Keywords

  • Algae
  • Biofuel
  • Chlamydomonas reinhardtii
  • Cw15
  • Flocculation
  • Methanol

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Flocculation of wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 by calcium and methanol. / Scholz, Matt; Hoshino, Takanori; Johnson, Daniel; Riley, Mark; Cuello, Joel.

In: Biomass and Bioenergy, Vol. 35, No. 12, 01.12.2011, p. 4835-4840.

Research output: Contribution to journalArticle

Scholz, Matt ; Hoshino, Takanori ; Johnson, Daniel ; Riley, Mark ; Cuello, Joel. / Flocculation of wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 by calcium and methanol. In: Biomass and Bioenergy. 2011 ; Vol. 35, No. 12. pp. 4835-4840.
@article{2e2c9ef31af243ccace23fe8c2dd8258,
title = "Flocculation of wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 by calcium and methanol",
abstract = "Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 ± 3{\%} of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 ± 2{\%} of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered that the addition of small amounts (<5{\%} v/v) of methanol could significantly reduce the calcium needed to achieve flocculation. Within 15 min after addition of 12 mM calcium chloride and 4.6{\%} (v/v) methanol, 83 ± 4{\%} of cw15 cells flocculated. Methanol is fully recoverable by distillation, and its use might enable flocculation without further water salinization when media calcium concentrations fall short of 15 mM. It was further shown that substrates for and/or products of cellular growth affected flocculation adversely. Nearly 81{\%} of cells flocculated from fresh medium compared to only 54{\%} in spent medium.",
keywords = "Algae, Biofuel, Chlamydomonas reinhardtii, Cw15, Flocculation, Methanol",
author = "Matt Scholz and Takanori Hoshino and Daniel Johnson and Mark Riley and Joel Cuello",
year = "2011",
month = "12",
day = "1",
doi = "10.1016/j.biombioe.2011.08.020",
language = "English (US)",
volume = "35",
pages = "4835--4840",
journal = "Biomass and Bioenergy",
issn = "0961-9534",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Flocculation of wall-deficient cells of Chlamydomonas reinhardtii mutant cw15 by calcium and methanol

AU - Scholz, Matt

AU - Hoshino, Takanori

AU - Johnson, Daniel

AU - Riley, Mark

AU - Cuello, Joel

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 ± 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 ± 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered that the addition of small amounts (<5% v/v) of methanol could significantly reduce the calcium needed to achieve flocculation. Within 15 min after addition of 12 mM calcium chloride and 4.6% (v/v) methanol, 83 ± 4% of cw15 cells flocculated. Methanol is fully recoverable by distillation, and its use might enable flocculation without further water salinization when media calcium concentrations fall short of 15 mM. It was further shown that substrates for and/or products of cellular growth affected flocculation adversely. Nearly 81% of cells flocculated from fresh medium compared to only 54% in spent medium.

AB - Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 ± 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 ± 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered that the addition of small amounts (<5% v/v) of methanol could significantly reduce the calcium needed to achieve flocculation. Within 15 min after addition of 12 mM calcium chloride and 4.6% (v/v) methanol, 83 ± 4% of cw15 cells flocculated. Methanol is fully recoverable by distillation, and its use might enable flocculation without further water salinization when media calcium concentrations fall short of 15 mM. It was further shown that substrates for and/or products of cellular growth affected flocculation adversely. Nearly 81% of cells flocculated from fresh medium compared to only 54% in spent medium.

KW - Algae

KW - Biofuel

KW - Chlamydomonas reinhardtii

KW - Cw15

KW - Flocculation

KW - Methanol

UR - http://www.scopus.com/inward/record.url?scp=82155166288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82155166288&partnerID=8YFLogxK

U2 - 10.1016/j.biombioe.2011.08.020

DO - 10.1016/j.biombioe.2011.08.020

M3 - Article

VL - 35

SP - 4835

EP - 4840

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

SN - 0961-9534

IS - 12

ER -