Flexibility difference between double-stranded RNA and DNA as revealed by gel electrophoresis

M. A. Livshits, O. A. Amosova, Yuri L Lyubchenko

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A systematic study of agarose gel electrophoresis of double-stranded RNA in the kilobase range of sizes was performed. The dsRNA to dsDNA relative mobility was found to depend on gel concentration: in low density gels RNA moves slower and in high density gels - faster than DNA of the same molecular size. The electrophoretic differences were interpreted within the reptation theory to be mainly due to the molecular stiffness differences. The dsRNA persistence length was roughly estimated to be about twice as great as that of DNA.

Original languageEnglish (US)
Pages (from-to)1237-1249
Number of pages13
JournalJournal of Biomolecular Structure and Dynamics
Volume7
Issue number6
DOIs
StatePublished - Jan 1 1990

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Double-Stranded RNA
Electrophoresis
Gels
DNA
Agar Gel Electrophoresis
RNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Flexibility difference between double-stranded RNA and DNA as revealed by gel electrophoresis. / Livshits, M. A.; Amosova, O. A.; Lyubchenko, Yuri L.

In: Journal of Biomolecular Structure and Dynamics, Vol. 7, No. 6, 01.01.1990, p. 1237-1249.

Research output: Contribution to journalArticle

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