Fixing coccidian oocysts is not an adequate solution to the problem of preserving protozoan type material

D. W. Duszynski, Scott L Gardner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fresh (36 days old) sporulated oocytes of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K2Cr2O7. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. The parameters examined were lengths and widths of oocysts and sporocysts, percent sporulation (%S), and percent crenation (%C) of oocysts and sporocysts. The highest destruction (%S and %C) occurred in oocysts exposed to glutaraldehyde and Karnovsky's fixatives where 100% of both oocysts and sporocysts crenated and only 8% and 48%, respectively, remained sporulated. Of the oocysts in paraformaldehyde, 93% remained sporulated, but 95% of these oocysts and 100% of the sporocysts crenated. In Bouin's solution, 75% of the oocysts were intact structurally, but of these, only 60% were still sporulated with 70% of their sporocysts crenated. Oocysts preserved in 70% EtOH were 80% intact and 70% remained sporulated, but nearly 60% of their sporocysts collapsed even though the oocyst walls were intact. Oocysts preserved in 10% buffered formalin maintained structural integrity but had lower numbers of sporulated oocysts (84%) and greater numbers of crenated oocysts (18%) than control oocysts maintained in the dichromate solution (95% and 0%, respectively).

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
JournalJournal of Parasitology
Volume77
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

sporulation
Oocysts
oocysts
fixation
Protozoa
ethanol
oil
sporocysts (Protozoa)
glutaraldehyde
effect
material
parameter
formalin
Eimeria nieschulzi
Glutaral
Formaldehyde
water
Potassium Dichromate
Eimeria
Fixatives

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Fixing coccidian oocysts is not an adequate solution to the problem of preserving protozoan type material. / Duszynski, D. W.; Gardner, Scott L.

In: Journal of Parasitology, Vol. 77, No. 1, 01.01.1991, p. 52-57.

Research output: Contribution to journalArticle

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