First results of digital topography applied to macromolecular crystals

Jeffrey J. Lovelace, Alexei S. Soares, Henry D. Bellamy, Robert M. Sweet, Edward H. Snell, Gloria E Borgstahl

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An inexpensive digital CCD camera was used to record X-ray topographs directly from large imperfect crystals of cubic insulin. The topographs recorded were not as detailed as those which can be measured with film or emulsion plates, but do show great promise. Six reflections were recorded using a set of finely spaced stills encompassing the rocking curve of each reflection. A complete topographic reflection profile could be digitally imaged in minutes. Interesting and complex internal structure was observed by this technique. The CCD chip used in the camera has anti-blooming circuitry and produced good data quality, even when pixels became overloaded.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
JournalJournal of Applied Crystallography
Volume37
Issue number3
DOIs
StatePublished - Jun 1 2004

Fingerprint

Emulsions
Topography
topography
X-Rays
Insulin
Crystals
stills
crystals
insulin
digital cameras
Digital cameras
CCD cameras
Charge coupled devices
emulsions
charge coupled devices
Pixels
Cameras
pixels
cameras
chips

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

First results of digital topography applied to macromolecular crystals. / Lovelace, Jeffrey J.; Soares, Alexei S.; Bellamy, Henry D.; Sweet, Robert M.; Snell, Edward H.; Borgstahl, Gloria E.

In: Journal of Applied Crystallography, Vol. 37, No. 3, 01.06.2004, p. 481-485.

Research output: Contribution to journalArticle

Lovelace, Jeffrey J. ; Soares, Alexei S. ; Bellamy, Henry D. ; Sweet, Robert M. ; Snell, Edward H. ; Borgstahl, Gloria E. / First results of digital topography applied to macromolecular crystals. In: Journal of Applied Crystallography. 2004 ; Vol. 37, No. 3. pp. 481-485.
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