First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas

André Luis Costa-da-Silva, Rafaella Sayuri Ioshino, Vivian Petersen, Antonio Fernando Lima, Marielton dos Passos Cunha, Michael R. Wiley, Jason T. Ladner, Karla Prieto, Gustavo Palacios, Danuza Duarte Costa, Lincoln Suesdek, Paolo Marinho de Andrade Zanotto, Margareth Lara Capurro

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: The worldwide expansion of new emergent arboviruses such as Chikungunya and Zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. This knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. Although the first autochthonous transmission of Chikungunya virus was described in 2014 in the north of Brazil, the main outbreaks were reported in 2015 and 2016 in the northeast of Brazil. Methodology/Principal findings: During 5 days of February 2016, we collected mosquitoes in homes of 6 neighborhoods of Aracaju city, the capital of Sergipe state. Four mosquito species were identified but Culex quinquefasciatus and Aedes aegypti were the most abundant. Field-caught mosquitoes were tested for Chikungunya (CHIKV), Zika (ZIKV) and Dengue viruses (DENV) by qRT-PCR and one CHIKV-infected Ae. aegypti female was detected. The complete sequence of CHIKV genome was obtained from this sample and phylogenetic analysis revealed that this isolate belongs to the East-Central-South-African (ECSA) genotype. Conclusions: Our study describes the first identification of a naturally CHIKV-infected Ae. aegypti in Brazil and the first report of a CHIKV from ECSA genotype identified in this species in the Americas. These findings support the notion of Ae. aegypti being a vector involved in CHIKV outbreaks in northeast of Brazil.

Original languageEnglish (US)
Article numbere0005630
JournalPLoS neglected tropical diseases
Volume11
Issue number6
DOIs
StatePublished - Jun 14 2017

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Chikungunya virus
Aedes
Culicidae
Brazil
Genotype
Disease Outbreaks
Arboviruses
Species Specificity
Culex
Dengue Virus
Infectious Disease Transmission
Genome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Costa-da-Silva, A. L., Ioshino, R. S., Petersen, V., Lima, A. F., Cunha, M. D. P., Wiley, M. R., ... Capurro, M. L. (2017). First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas. PLoS neglected tropical diseases, 11(6), [e0005630]. https://doi.org/10.1371/journal.pntd.0005630

First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas. / Costa-da-Silva, André Luis; Ioshino, Rafaella Sayuri; Petersen, Vivian; Lima, Antonio Fernando; Cunha, Marielton dos Passos; Wiley, Michael R.; Ladner, Jason T.; Prieto, Karla; Palacios, Gustavo; Costa, Danuza Duarte; Suesdek, Lincoln; Zanotto, Paolo Marinho de Andrade; Capurro, Margareth Lara.

In: PLoS neglected tropical diseases, Vol. 11, No. 6, e0005630, 14.06.2017.

Research output: Contribution to journalArticle

Costa-da-Silva, AL, Ioshino, RS, Petersen, V, Lima, AF, Cunha, MDP, Wiley, MR, Ladner, JT, Prieto, K, Palacios, G, Costa, DD, Suesdek, L, Zanotto, PMDA & Capurro, ML 2017, 'First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas', PLoS neglected tropical diseases, vol. 11, no. 6, e0005630. https://doi.org/10.1371/journal.pntd.0005630
Costa-da-Silva, André Luis ; Ioshino, Rafaella Sayuri ; Petersen, Vivian ; Lima, Antonio Fernando ; Cunha, Marielton dos Passos ; Wiley, Michael R. ; Ladner, Jason T. ; Prieto, Karla ; Palacios, Gustavo ; Costa, Danuza Duarte ; Suesdek, Lincoln ; Zanotto, Paolo Marinho de Andrade ; Capurro, Margareth Lara. / First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas. In: PLoS neglected tropical diseases. 2017 ; Vol. 11, No. 6.
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AU - Lima, Antonio Fernando

AU - Cunha, Marielton dos Passos

AU - Wiley, Michael R.

AU - Ladner, Jason T.

AU - Prieto, Karla

AU - Palacios, Gustavo

AU - Costa, Danuza Duarte

AU - Suesdek, Lincoln

AU - Zanotto, Paolo Marinho de Andrade

AU - Capurro, Margareth Lara

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N2 - Background: The worldwide expansion of new emergent arboviruses such as Chikungunya and Zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. This knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. Although the first autochthonous transmission of Chikungunya virus was described in 2014 in the north of Brazil, the main outbreaks were reported in 2015 and 2016 in the northeast of Brazil. Methodology/Principal findings: During 5 days of February 2016, we collected mosquitoes in homes of 6 neighborhoods of Aracaju city, the capital of Sergipe state. Four mosquito species were identified but Culex quinquefasciatus and Aedes aegypti were the most abundant. Field-caught mosquitoes were tested for Chikungunya (CHIKV), Zika (ZIKV) and Dengue viruses (DENV) by qRT-PCR and one CHIKV-infected Ae. aegypti female was detected. The complete sequence of CHIKV genome was obtained from this sample and phylogenetic analysis revealed that this isolate belongs to the East-Central-South-African (ECSA) genotype. Conclusions: Our study describes the first identification of a naturally CHIKV-infected Ae. aegypti in Brazil and the first report of a CHIKV from ECSA genotype identified in this species in the Americas. These findings support the notion of Ae. aegypti being a vector involved in CHIKV outbreaks in northeast of Brazil.

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