First evidence of antibodies against lloviu virus in schreiber’s bent-winged insectivorous bats demonstrate a wide circulation of the virus in spain

Eva Ramírez De Arellano, Mariano Sanchez-Lockhart, Maria J. Perteguer, Maggie Bartlett, Marta Ortiz, Pamela Campioli, Ana Hernández, Jeanette Gonzalez, Karla Garcia, Manolo Ramos, Miguel Ángel Jiménez-Clavero, Antonio Tenorio, Mª Paz Sánchez-Seco, Félix González, Juan Emilio Echevarría, Gustavo Palacios, Anabel Negred

Research output: Contribution to journalArticle

Abstract

Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of.

Original languageEnglish (US)
Article number360
JournalViruses
Volume11
Issue number4
DOIs
StatePublished - Apr 2019

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Spain
Viruses
Antibodies
Epitopes
Seroepidemiologic Studies
Serology
Serum
Bacteriophages
Virulence
Proteins

Keywords

  • Bats
  • Human
  • Lloviu virus
  • Prevalence
  • Serology

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

First evidence of antibodies against lloviu virus in schreiber’s bent-winged insectivorous bats demonstrate a wide circulation of the virus in spain. / De Arellano, Eva Ramírez; Sanchez-Lockhart, Mariano; Perteguer, Maria J.; Bartlett, Maggie; Ortiz, Marta; Campioli, Pamela; Hernández, Ana; Gonzalez, Jeanette; Garcia, Karla; Ramos, Manolo; Jiménez-Clavero, Miguel Ángel; Tenorio, Antonio; Sánchez-Seco, Mª Paz; González, Félix; Echevarría, Juan Emilio; Palacios, Gustavo; Negred, Anabel.

In: Viruses, Vol. 11, No. 4, 360, 04.2019.

Research output: Contribution to journalArticle

De Arellano, ER, Sanchez-Lockhart, M, Perteguer, MJ, Bartlett, M, Ortiz, M, Campioli, P, Hernández, A, Gonzalez, J, Garcia, K, Ramos, M, Jiménez-Clavero, MÁ, Tenorio, A, Sánchez-Seco, MP, González, F, Echevarría, JE, Palacios, G & Negred, A 2019, 'First evidence of antibodies against lloviu virus in schreiber’s bent-winged insectivorous bats demonstrate a wide circulation of the virus in spain', Viruses, vol. 11, no. 4, 360. https://doi.org/10.3390/v11040360
De Arellano, Eva Ramírez ; Sanchez-Lockhart, Mariano ; Perteguer, Maria J. ; Bartlett, Maggie ; Ortiz, Marta ; Campioli, Pamela ; Hernández, Ana ; Gonzalez, Jeanette ; Garcia, Karla ; Ramos, Manolo ; Jiménez-Clavero, Miguel Ángel ; Tenorio, Antonio ; Sánchez-Seco, Mª Paz ; González, Félix ; Echevarría, Juan Emilio ; Palacios, Gustavo ; Negred, Anabel. / First evidence of antibodies against lloviu virus in schreiber’s bent-winged insectivorous bats demonstrate a wide circulation of the virus in spain. In: Viruses. 2019 ; Vol. 11, No. 4.
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abstract = "Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5{\%}). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of.",
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AU - Bartlett, Maggie

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AU - Jiménez-Clavero, Miguel Ángel

AU - Tenorio, Antonio

AU - Sánchez-Seco, Mª Paz

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AU - Negred, Anabel

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