Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells

Katarzyna Dziewanowska, Joseph M. Patti, Claudia F. Deobald, Kenneth W Bayles, William R. Trumble, Gregory A. Bohach

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Staphylococcus aureus expresses several surface proteins that promote adherence to host cell extracellular matrix proteins, including fibronectin (Fn). Since this organism has recently been shown to be internalized by nonprofessional phagocytes, a process that typically requires high-affinity binding to host cell receptors, we investigated the role of its Fn binding proteins (FnBPs) and other surface proteins in internalization by the bovine mammary gland epithelial cell line (MAC-T). Efficient internaiization of S. aureus 8325-4 required expression of FnBPs; an isogenic mutant (DU5883), not expressing FnBPs, was reduced by more than 95% in its ability to invade MAC-T cells. Moreover, D3, a synthetic peptide derived from the ligand binding domain of FnBP, inhibited the internalization of the 8325-4 strain in a dose- dependent fashion and the efficiency of staphylococcal internalization was partially correlated with Fn binding ability. Interestingly, Fn also inhibited the internalization and adherence of S. aureus 8325-4 in a dose- dependent manner. In contrast to internalization, adherence of DU5883 to MAC- T was reduced by only approximately 40%. Suggesting that surface binding proteins, other than FnBPs, can mediate bacterial adherence to cells. Adherence via these proteins, however, does not necessarily result in internalization of the staphylococci. An inhibitor of protein tyrosine kinase, genistein, reduced MAC-T internalization of S. aureus by 95%, indicating a requirement for a host signal transduction system in this process. Taken together, these results indicate that S. aureus invades nonprofessional phagocytes by a mechanism requiting interaction between FnBP and the host cell, leading to signal transduction and subsequent rearrangement of the host cell cytoskeleton.

Original languageEnglish (US)
Pages (from-to)4673-4678
Number of pages6
JournalInfection and Immunity
Volume67
Issue number9
StatePublished - Sep 1 1999

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Fibronectins
Protein-Tyrosine Kinases
Staphylococcus aureus
Carrier Proteins
Epithelial Cells
Membrane Proteins
Phagocytes
Signal Transduction
Genistein
Extracellular Matrix Proteins
Human Mammary Glands
Cytoskeleton
Staphylococcus
Ligands
T-Lymphocytes
Cell Line
Peptides
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells. / Dziewanowska, Katarzyna; Patti, Joseph M.; Deobald, Claudia F.; Bayles, Kenneth W; Trumble, William R.; Bohach, Gregory A.

In: Infection and Immunity, Vol. 67, No. 9, 01.09.1999, p. 4673-4678.

Research output: Contribution to journalArticle

Dziewanowska, Katarzyna ; Patti, Joseph M. ; Deobald, Claudia F. ; Bayles, Kenneth W ; Trumble, William R. ; Bohach, Gregory A. / Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells. In: Infection and Immunity. 1999 ; Vol. 67, No. 9. pp. 4673-4678.
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abstract = "Staphylococcus aureus expresses several surface proteins that promote adherence to host cell extracellular matrix proteins, including fibronectin (Fn). Since this organism has recently been shown to be internalized by nonprofessional phagocytes, a process that typically requires high-affinity binding to host cell receptors, we investigated the role of its Fn binding proteins (FnBPs) and other surface proteins in internalization by the bovine mammary gland epithelial cell line (MAC-T). Efficient internaiization of S. aureus 8325-4 required expression of FnBPs; an isogenic mutant (DU5883), not expressing FnBPs, was reduced by more than 95{\%} in its ability to invade MAC-T cells. Moreover, D3, a synthetic peptide derived from the ligand binding domain of FnBP, inhibited the internalization of the 8325-4 strain in a dose- dependent fashion and the efficiency of staphylococcal internalization was partially correlated with Fn binding ability. Interestingly, Fn also inhibited the internalization and adherence of S. aureus 8325-4 in a dose- dependent manner. In contrast to internalization, adherence of DU5883 to MAC- T was reduced by only approximately 40{\%}. Suggesting that surface binding proteins, other than FnBPs, can mediate bacterial adherence to cells. Adherence via these proteins, however, does not necessarily result in internalization of the staphylococci. An inhibitor of protein tyrosine kinase, genistein, reduced MAC-T internalization of S. aureus by 95{\%}, indicating a requirement for a host signal transduction system in this process. Taken together, these results indicate that S. aureus invades nonprofessional phagocytes by a mechanism requiting interaction between FnBP and the host cell, leading to signal transduction and subsequent rearrangement of the host cell cytoskeleton.",
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