Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture

Yunkui Zhu, C. Magnus Sköld, Xiang-de Liu, Hangjun Wang, Tadashi Kohyama, Fu Qiang Wen, Ronald F. Ertl, Stephen I. Rennard

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels. Methods: Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Results: Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes (P<0.01). However, with extended co-culture, contraction of the collagen gels was greatly augmented (P<0.01). In addition, with extended co-culture, degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation (P<0.01). Prostaglandin E2 production was significantly increased by co-culture and its presence attenuated collagen degradation. Conclusion: The current study, therefore, demonstrates that interaction between monocytes and fibroblasts can contract and degrade extracellular matrix in extended culture.

Original languageEnglish (US)
Pages (from-to)295-299
Number of pages5
JournalRespiratory Research
Volume2
Issue number5
DOIs
StatePublished - Dec 1 2001

Fingerprint

Coculture Techniques
Monocytes
Collagen
Fibroblasts
Gels
Macrophages
Leukocyte Elastase
Collagen Type I
Dinoprostone
Extracellular Matrix
Lung

Keywords

  • Collagen degradation
  • IL-1
  • Lung fibroblasts
  • Monocytes
  • Neutrophil elastase
  • PGE
  • TNF-α

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture. / Zhu, Yunkui; Sköld, C. Magnus; Liu, Xiang-de; Wang, Hangjun; Kohyama, Tadashi; Wen, Fu Qiang; Ertl, Ronald F.; Rennard, Stephen I.

In: Respiratory Research, Vol. 2, No. 5, 01.12.2001, p. 295-299.

Research output: Contribution to journalArticle

Zhu, Y, Sköld, CM, Liu, X, Wang, H, Kohyama, T, Wen, FQ, Ertl, RF & Rennard, SI 2001, 'Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture', Respiratory Research, vol. 2, no. 5, pp. 295-299. https://doi.org/10.1186/rr72
Zhu, Yunkui ; Sköld, C. Magnus ; Liu, Xiang-de ; Wang, Hangjun ; Kohyama, Tadashi ; Wen, Fu Qiang ; Ertl, Ronald F. ; Rennard, Stephen I. / Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture. In: Respiratory Research. 2001 ; Vol. 2, No. 5. pp. 295-299.
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AU - Kohyama, Tadashi

AU - Wen, Fu Qiang

AU - Ertl, Ronald F.

AU - Rennard, Stephen I.

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N2 - Background: Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels. Methods: Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Results: Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes (P<0.01). However, with extended co-culture, contraction of the collagen gels was greatly augmented (P<0.01). In addition, with extended co-culture, degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation (P<0.01). Prostaglandin E2 production was significantly increased by co-culture and its presence attenuated collagen degradation. Conclusion: The current study, therefore, demonstrates that interaction between monocytes and fibroblasts can contract and degrade extracellular matrix in extended culture.

AB - Background: Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels. Methods: Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Results: Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes (P<0.01). However, with extended co-culture, contraction of the collagen gels was greatly augmented (P<0.01). In addition, with extended co-culture, degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation (P<0.01). Prostaglandin E2 production was significantly increased by co-culture and its presence attenuated collagen degradation. Conclusion: The current study, therefore, demonstrates that interaction between monocytes and fibroblasts can contract and degrade extracellular matrix in extended culture.

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