Fecal prevalence of Escherichia coli O157, Salmonella, Listeria, and bacteriophage infecting E. coli O157:H7 in feedlot cattle in the southern plains region of the United States

T. R. Callaway, T. S. Edrington, A. D. Brabban, J. E. Keen, R. C. Anderson, M. L. Rossman, M. J. Engler, K. J. Genovese, B. L. Gwartney, J. O. Reagan, T. L. Poole, R. B. Harvey, E. M. Kutter, D. J. Nisbet

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Escherichia coli O157:H7, Salmonella, and Listeria are foodborne pathogens of critical importance that often colonize cattle. E. coli O157:H7 can be specifically killed by lytic bacteriophage, and lytic bacteriophage treatment has been suggested as a pre-harvest intervention strategy to reduce foodborne pathogens in cattle. To date, no systematic approach to determine the incidence of E. coli O157:H7-infecting lytic bacteriophage has been published. Therefore, the current study was designed to determine (1) the incidence of E. coli O157, Salmonella spp., and Listeria and (2) the incidence of E. coli O157:H7-infecting bacteriophage in the feces of feedlot steers in commercial feedlots in the United States. Fecal samples (n = 60) were collected from four feedlots in two Southern Great Plains states (total n = 240 fecal samples). Salmonella and E. coli O157:H7 were found in 3.8% and 11.7% of the fecal samples, respectively. Bacteriophage targeting E. coli O157:H7 were found in all four feedlots, in 15% of the individual fecal samples, and in 55% of the cattle pens. Our results indicate that such bacteriophage are widespread in feedlot cattle, suggesting that further research into the ecological role of bacteriophage in the gastrointestinal tract is needed.

Original languageEnglish (US)
Pages (from-to)234-244
Number of pages11
JournalFoodborne pathogens and disease
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2006

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Salmonella Phages
Listeria
Escherichia coli O157
feedlots
bacteriophages
Bacteriophages
Salmonella
cattle
food pathogens
incidence
Incidence
sampling
Feces
gastrointestinal system
Gastrointestinal Tract
feces

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Cite this

Fecal prevalence of Escherichia coli O157, Salmonella, Listeria, and bacteriophage infecting E. coli O157:H7 in feedlot cattle in the southern plains region of the United States. / Callaway, T. R.; Edrington, T. S.; Brabban, A. D.; Keen, J. E.; Anderson, R. C.; Rossman, M. L.; Engler, M. J.; Genovese, K. J.; Gwartney, B. L.; Reagan, J. O.; Poole, T. L.; Harvey, R. B.; Kutter, E. M.; Nisbet, D. J.

In: Foodborne pathogens and disease, Vol. 3, No. 3, 01.09.2006, p. 234-244.

Research output: Contribution to journalArticle

Callaway, TR, Edrington, TS, Brabban, AD, Keen, JE, Anderson, RC, Rossman, ML, Engler, MJ, Genovese, KJ, Gwartney, BL, Reagan, JO, Poole, TL, Harvey, RB, Kutter, EM & Nisbet, DJ 2006, 'Fecal prevalence of Escherichia coli O157, Salmonella, Listeria, and bacteriophage infecting E. coli O157:H7 in feedlot cattle in the southern plains region of the United States', Foodborne pathogens and disease, vol. 3, no. 3, pp. 234-244. https://doi.org/10.1089/fpd.2006.3.234
Callaway, T. R. ; Edrington, T. S. ; Brabban, A. D. ; Keen, J. E. ; Anderson, R. C. ; Rossman, M. L. ; Engler, M. J. ; Genovese, K. J. ; Gwartney, B. L. ; Reagan, J. O. ; Poole, T. L. ; Harvey, R. B. ; Kutter, E. M. ; Nisbet, D. J. / Fecal prevalence of Escherichia coli O157, Salmonella, Listeria, and bacteriophage infecting E. coli O157:H7 in feedlot cattle in the southern plains region of the United States. In: Foodborne pathogens and disease. 2006 ; Vol. 3, No. 3. pp. 234-244.
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