Feasibility of using capillary zone electrophoresis with photometric detection for the trace level detection of bromate in drinking water

Tammy Gries, Emily Sitorius, Anne Giesecke, Vicki L Schlegel

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4 Citations (Scopus)

Abstract

The feasibility of applying a capillary zone electrophoretic (CZE) method for the trace analysis of bromate, a suspect human carcinogen, in drinking water was studied. Using a bare fused-silica capillary (75 μm inner diameter) coupled with indirect ultraviolet detection (200 nm), 0.25 mM cetyltrimethylammonium bromide, 5% methanol and 5.0 mM phthalate at pH 5.0-5.2, an electrokinetic injection of 15 kV and 10 s, a separation voltage of 18 kV (negative polarity) and a capillary temperature of 15°C, bromate was detected in high purity water at ≤10 μl-1 The method was applied to three bottled water sources and to local municipal water. If needed, additional sample-handling steps, consisting of an off-line pre-concentration step and pH adjustment to 5.5, was used to improve detection limits (from a high of 400 μg l-1 to ≤10 μg l-1) and baseline noise. Signal-to-noise ratios also increased by adding sodium phosphate (1.1 μg ml-1) to all sources prior to analysis. Although the CZE method was capable of detecting bromate at levels ≤10 μl-1 with an analysis time of 8-9 min, high variability (≥10% relative standard deviation) precluded its application to some of sources without further method development. Nonetheless, this method could serve as the basis for the detection of bromate to specific water sources with minimal or no optimization.

Original languageEnglish (US)
Pages (from-to)1318-1327
Number of pages10
JournalFood Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
Volume25
Issue number11
DOIs
StatePublished - Dec 1 2008

Fingerprint

bromates
Bromates
capillary zone electrophoresis
Capillary Electrophoresis
Electrophoresis
Drinking Water
drinking water
Water
Trace analysis
bottled water
public water supply
Fused silica
methodology
phthalates
sodium phosphate
Carcinogens
Methanol
Signal-To-Noise Ratio
carcinogens
Signal to noise ratio

Keywords

  • Bromate
  • Chromatography/electrophoresis
  • Method validation
  • Water

ASJC Scopus subject areas

  • Food Science
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Toxicology
  • Chemistry(all)

Cite this

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title = "Feasibility of using capillary zone electrophoresis with photometric detection for the trace level detection of bromate in drinking water",
abstract = "The feasibility of applying a capillary zone electrophoretic (CZE) method for the trace analysis of bromate, a suspect human carcinogen, in drinking water was studied. Using a bare fused-silica capillary (75 μm inner diameter) coupled with indirect ultraviolet detection (200 nm), 0.25 mM cetyltrimethylammonium bromide, 5{\%} methanol and 5.0 mM phthalate at pH 5.0-5.2, an electrokinetic injection of 15 kV and 10 s, a separation voltage of 18 kV (negative polarity) and a capillary temperature of 15°C, bromate was detected in high purity water at ≤10 μl-1 The method was applied to three bottled water sources and to local municipal water. If needed, additional sample-handling steps, consisting of an off-line pre-concentration step and pH adjustment to 5.5, was used to improve detection limits (from a high of 400 μg l-1 to ≤10 μg l-1) and baseline noise. Signal-to-noise ratios also increased by adding sodium phosphate (1.1 μg ml-1) to all sources prior to analysis. Although the CZE method was capable of detecting bromate at levels ≤10 μl-1 with an analysis time of 8-9 min, high variability (≥10{\%} relative standard deviation) precluded its application to some of sources without further method development. Nonetheless, this method could serve as the basis for the detection of bromate to specific water sources with minimal or no optimization.",
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N2 - The feasibility of applying a capillary zone electrophoretic (CZE) method for the trace analysis of bromate, a suspect human carcinogen, in drinking water was studied. Using a bare fused-silica capillary (75 μm inner diameter) coupled with indirect ultraviolet detection (200 nm), 0.25 mM cetyltrimethylammonium bromide, 5% methanol and 5.0 mM phthalate at pH 5.0-5.2, an electrokinetic injection of 15 kV and 10 s, a separation voltage of 18 kV (negative polarity) and a capillary temperature of 15°C, bromate was detected in high purity water at ≤10 μl-1 The method was applied to three bottled water sources and to local municipal water. If needed, additional sample-handling steps, consisting of an off-line pre-concentration step and pH adjustment to 5.5, was used to improve detection limits (from a high of 400 μg l-1 to ≤10 μg l-1) and baseline noise. Signal-to-noise ratios also increased by adding sodium phosphate (1.1 μg ml-1) to all sources prior to analysis. Although the CZE method was capable of detecting bromate at levels ≤10 μl-1 with an analysis time of 8-9 min, high variability (≥10% relative standard deviation) precluded its application to some of sources without further method development. Nonetheless, this method could serve as the basis for the detection of bromate to specific water sources with minimal or no optimization.

AB - The feasibility of applying a capillary zone electrophoretic (CZE) method for the trace analysis of bromate, a suspect human carcinogen, in drinking water was studied. Using a bare fused-silica capillary (75 μm inner diameter) coupled with indirect ultraviolet detection (200 nm), 0.25 mM cetyltrimethylammonium bromide, 5% methanol and 5.0 mM phthalate at pH 5.0-5.2, an electrokinetic injection of 15 kV and 10 s, a separation voltage of 18 kV (negative polarity) and a capillary temperature of 15°C, bromate was detected in high purity water at ≤10 μl-1 The method was applied to three bottled water sources and to local municipal water. If needed, additional sample-handling steps, consisting of an off-line pre-concentration step and pH adjustment to 5.5, was used to improve detection limits (from a high of 400 μg l-1 to ≤10 μg l-1) and baseline noise. Signal-to-noise ratios also increased by adding sodium phosphate (1.1 μg ml-1) to all sources prior to analysis. Although the CZE method was capable of detecting bromate at levels ≤10 μl-1 with an analysis time of 8-9 min, high variability (≥10% relative standard deviation) precluded its application to some of sources without further method development. Nonetheless, this method could serve as the basis for the detection of bromate to specific water sources with minimal or no optimization.

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