FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques

Rajnish S. Dave, Ravi K. Sharma, Roshell R. Muir, Elias Haddad, Sanjeev Gumber, Francois Villinger, Artinder P. Nehra, Zafar K. Khan, Brian Wigdahl, Aftab A. Ansari, Siddappa Nagadenahalli Byrareddy, Pooja Jain

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cerebrospinal fluid (CSF) drains via the lymphatic drainage pathway. This lymphatic pathway connects the central nervous system (CNS) to the cervical lymph node (CLN). As the CSF drains to CLN via the dural and nasal lymphatics, T cells and antigen presenting cells pass along the channels from the subarachnoid space through the cribriform plate. Human immunodeficiency virus (HIV) may also egress from the CNS along this pathway. As a result, HIV egressing from the CNS may accumulate within the CLN. Towards this objective, we analyzed CLNs isolated from rhesus macaques that were chronically-infected with simian immunodeficiency virus (SIV). We detected significant accumulation of SIV within the CLNs. SIV virion trapping was observed on follicular dendritic cells (FDCs) localized within the follicular regions of CLNs. In addition, SIV antigens formed immune complexes when FDCs interacted with B cells within the germinal centers. Subsequent interaction of these B cells with CD4+ T follicular helper cells (TFHs) resulted in infection of the latter. Of note, 73% to 90% of the TFHs cells within CLNs were positive for SIV p27 antigen. As such, it appears that not only do the FDCs retain SIV they also transmit them (via B cells) to TFHs within these CLNs. This interaction results in infection of TFHs in the CLNs. Based on these observations, we infer that FDCs within the CLNs have a novel role in SIV entrapment with implications for viral trafficking.

Original languageEnglish (US)
Pages (from-to)204-218
Number of pages15
JournalJournal of Neuroimmune Pharmacology
Volume13
Issue number2
DOIs
StatePublished - Jun 1 2018

Fingerprint

Follicular Dendritic Cells
Simian Immunodeficiency Virus
Macaca mulatta
Cell Communication
Lymph Nodes
B-Lymphocytes
Central Nervous System
Cerebrospinal Fluid
Ethmoid Bone
HIV
Virus Release
Subarachnoid Space
Germinal Center
Viral Tumor Antigens
Antigen-Presenting Cells
Helper-Inducer T-Lymphocytes
Infection
Antigen-Antibody Complex
Nose
Virion

Keywords

  • CD4+ T follicular helper cells (TFHs)
  • Central nervous system (CNS)
  • Follicular dendritic cells (FDCs)
  • Human immunodeficiency virus (HIV)
  • Simian immunodeficiency virus (SIV)

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Dave, R. S., Sharma, R. K., Muir, R. R., Haddad, E., Gumber, S., Villinger, F., ... Jain, P. (2018). FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. Journal of Neuroimmune Pharmacology, 13(2), 204-218. https://doi.org/10.1007/s11481-017-9775-0

FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. / Dave, Rajnish S.; Sharma, Ravi K.; Muir, Roshell R.; Haddad, Elias; Gumber, Sanjeev; Villinger, Francois; Nehra, Artinder P.; Khan, Zafar K.; Wigdahl, Brian; Ansari, Aftab A.; Byrareddy, Siddappa Nagadenahalli; Jain, Pooja.

In: Journal of Neuroimmune Pharmacology, Vol. 13, No. 2, 01.06.2018, p. 204-218.

Research output: Contribution to journalArticle

Dave, RS, Sharma, RK, Muir, RR, Haddad, E, Gumber, S, Villinger, F, Nehra, AP, Khan, ZK, Wigdahl, B, Ansari, AA, Byrareddy, SN & Jain, P 2018, 'FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques', Journal of Neuroimmune Pharmacology, vol. 13, no. 2, pp. 204-218. https://doi.org/10.1007/s11481-017-9775-0
Dave, Rajnish S. ; Sharma, Ravi K. ; Muir, Roshell R. ; Haddad, Elias ; Gumber, Sanjeev ; Villinger, Francois ; Nehra, Artinder P. ; Khan, Zafar K. ; Wigdahl, Brian ; Ansari, Aftab A. ; Byrareddy, Siddappa Nagadenahalli ; Jain, Pooja. / FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. In: Journal of Neuroimmune Pharmacology. 2018 ; Vol. 13, No. 2. pp. 204-218.
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abstract = "Cerebrospinal fluid (CSF) drains via the lymphatic drainage pathway. This lymphatic pathway connects the central nervous system (CNS) to the cervical lymph node (CLN). As the CSF drains to CLN via the dural and nasal lymphatics, T cells and antigen presenting cells pass along the channels from the subarachnoid space through the cribriform plate. Human immunodeficiency virus (HIV) may also egress from the CNS along this pathway. As a result, HIV egressing from the CNS may accumulate within the CLN. Towards this objective, we analyzed CLNs isolated from rhesus macaques that were chronically-infected with simian immunodeficiency virus (SIV). We detected significant accumulation of SIV within the CLNs. SIV virion trapping was observed on follicular dendritic cells (FDCs) localized within the follicular regions of CLNs. In addition, SIV antigens formed immune complexes when FDCs interacted with B cells within the germinal centers. Subsequent interaction of these B cells with CD4+ T follicular helper cells (TFHs) resulted in infection of the latter. Of note, 73{\%} to 90{\%} of the TFHs cells within CLNs were positive for SIV p27 antigen. As such, it appears that not only do the FDCs retain SIV they also transmit them (via B cells) to TFHs within these CLNs. This interaction results in infection of TFHs in the CLNs. Based on these observations, we infer that FDCs within the CLNs have a novel role in SIV entrapment with implications for viral trafficking.",
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AU - Nehra, Artinder P.

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