Fatty acid transport by vectorial acylation in mammals: Roles played by different isoforms of rat long-chain acyl-CoA synthetases

Fumin Tong, Paul N. Black, Rosalind A. Coleman, Concetta C. DiRusso

Research output: Contribution to journalArticle

36 Scopus citations


Mammals express multiple isoforms of acyl-CoA synthetase (ACSL1 and ACSL3-6) in various tissues. These enzymes are essential for fatty acid metabolism providing activated intermediates for complex lipid synthesis, protein modification, and β-oxidation. Yeast in contrast express four major ACSLs, which have well-defined functions. Two, Faa1p and Faa4p, are specifically required for fatty acid transport by vectorial acylation. Four ACSLs from the rat were expressed in a yeast faa1Δ faa4Δ strain and their roles in fatty acid transport and trafficking characterized. All four restored ACS activity yet varied in substrate preference. ACSL1, 4, and 6 were able to rescue fatty acid transport activity and triglyceride synthesis. ACSL5, however, was unable to facilitate fatty acid transport despite conferring robust oleoyl-CoA synthetase activity. This is the first study evaluating the role of the mammalian ACSLs in fatty acid transport and supports a role for ACSL1, 4, and 6 in transport by vectorial acylation.

Original languageEnglish (US)
Pages (from-to)46-52
Number of pages7
JournalArchives of Biochemistry and Biophysics
Issue number1
Publication statusPublished - Mar 1 2006



  • Acyl-CoA synthetase
  • Fatty acid transport
  • Rat
  • Triglyceride synthesis
  • Vectorial acylation
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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