Fasting exacerbates and feeding diminishes LPS-induced liver injury in the rat

Sasha D. Adams, Benjamin A. Delano, Kenneth S. Helmer, David W Mercer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Introduction Enteral nutrition improves clinical outcomes. The effects of feeding on LPS induced liver injury are unknown. We hypothesized that feeding would attenuate liver injury from LPS. Methods Fasted or fed rats were given LPS (20 mg/kg ip) or saline for 5 h and sacrificed. Serum aminotransferases and cytokines (immunoassay) were measured. Oxidative stress protein (iNOS, COX2, and HO1) assessments (Western immunoblot) were also obtained. Results In fasted rats, LPS significantly increased serum aminotransferase levels, enhanced hepatic COX2, iNOS, and HO1 immunoreactivity, and increased serum cytokine levels when compared to controls. While feeding diminished liver enzymes, attenuated expression of COX2 and iNOS, and blunted production of pro-inflammatory cytokines, it did not modulate LPS-induced expression of the anti-inflammatory markers HO1 and IL-10. Conclusion These data suggest that feeding decreases liver injury by attenuating expression of pro-inflammatory mediators while maintaining expression of anti-inflammatory mediators, both systemically and locally.

Original languageEnglish (US)
Pages (from-to)767-773
Number of pages7
JournalDigestive Diseases and Sciences
Volume54
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Fasting
Liver
Wounds and Injuries
Cytokines
Transaminases
Anti-Inflammatory Agents
Serum
Enteral Nutrition
Heat-Shock Proteins
Immunoassay
Interleukin-10
Oxidative Stress
Western Blotting
Enzymes

Keywords

  • Cytokines
  • Enteral nutrition
  • Lipopolysaccharide
  • Liver
  • Stress proteins

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Fasting exacerbates and feeding diminishes LPS-induced liver injury in the rat. / Adams, Sasha D.; Delano, Benjamin A.; Helmer, Kenneth S.; Mercer, David W.

In: Digestive Diseases and Sciences, Vol. 54, No. 4, 01.04.2009, p. 767-773.

Research output: Contribution to journalArticle

Adams, Sasha D. ; Delano, Benjamin A. ; Helmer, Kenneth S. ; Mercer, David W. / Fasting exacerbates and feeding diminishes LPS-induced liver injury in the rat. In: Digestive Diseases and Sciences. 2009 ; Vol. 54, No. 4. pp. 767-773.
@article{188137a60d154d408bc16574c2d335a8,
title = "Fasting exacerbates and feeding diminishes LPS-induced liver injury in the rat",
abstract = "Introduction Enteral nutrition improves clinical outcomes. The effects of feeding on LPS induced liver injury are unknown. We hypothesized that feeding would attenuate liver injury from LPS. Methods Fasted or fed rats were given LPS (20 mg/kg ip) or saline for 5 h and sacrificed. Serum aminotransferases and cytokines (immunoassay) were measured. Oxidative stress protein (iNOS, COX2, and HO1) assessments (Western immunoblot) were also obtained. Results In fasted rats, LPS significantly increased serum aminotransferase levels, enhanced hepatic COX2, iNOS, and HO1 immunoreactivity, and increased serum cytokine levels when compared to controls. While feeding diminished liver enzymes, attenuated expression of COX2 and iNOS, and blunted production of pro-inflammatory cytokines, it did not modulate LPS-induced expression of the anti-inflammatory markers HO1 and IL-10. Conclusion These data suggest that feeding decreases liver injury by attenuating expression of pro-inflammatory mediators while maintaining expression of anti-inflammatory mediators, both systemically and locally.",
keywords = "Cytokines, Enteral nutrition, Lipopolysaccharide, Liver, Stress proteins",
author = "Adams, {Sasha D.} and Delano, {Benjamin A.} and Helmer, {Kenneth S.} and Mercer, {David W}",
year = "2009",
month = "4",
day = "1",
doi = "10.1007/s10620-008-0425-5",
language = "English (US)",
volume = "54",
pages = "767--773",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Fasting exacerbates and feeding diminishes LPS-induced liver injury in the rat

AU - Adams, Sasha D.

AU - Delano, Benjamin A.

AU - Helmer, Kenneth S.

AU - Mercer, David W

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Introduction Enteral nutrition improves clinical outcomes. The effects of feeding on LPS induced liver injury are unknown. We hypothesized that feeding would attenuate liver injury from LPS. Methods Fasted or fed rats were given LPS (20 mg/kg ip) or saline for 5 h and sacrificed. Serum aminotransferases and cytokines (immunoassay) were measured. Oxidative stress protein (iNOS, COX2, and HO1) assessments (Western immunoblot) were also obtained. Results In fasted rats, LPS significantly increased serum aminotransferase levels, enhanced hepatic COX2, iNOS, and HO1 immunoreactivity, and increased serum cytokine levels when compared to controls. While feeding diminished liver enzymes, attenuated expression of COX2 and iNOS, and blunted production of pro-inflammatory cytokines, it did not modulate LPS-induced expression of the anti-inflammatory markers HO1 and IL-10. Conclusion These data suggest that feeding decreases liver injury by attenuating expression of pro-inflammatory mediators while maintaining expression of anti-inflammatory mediators, both systemically and locally.

AB - Introduction Enteral nutrition improves clinical outcomes. The effects of feeding on LPS induced liver injury are unknown. We hypothesized that feeding would attenuate liver injury from LPS. Methods Fasted or fed rats were given LPS (20 mg/kg ip) or saline for 5 h and sacrificed. Serum aminotransferases and cytokines (immunoassay) were measured. Oxidative stress protein (iNOS, COX2, and HO1) assessments (Western immunoblot) were also obtained. Results In fasted rats, LPS significantly increased serum aminotransferase levels, enhanced hepatic COX2, iNOS, and HO1 immunoreactivity, and increased serum cytokine levels when compared to controls. While feeding diminished liver enzymes, attenuated expression of COX2 and iNOS, and blunted production of pro-inflammatory cytokines, it did not modulate LPS-induced expression of the anti-inflammatory markers HO1 and IL-10. Conclusion These data suggest that feeding decreases liver injury by attenuating expression of pro-inflammatory mediators while maintaining expression of anti-inflammatory mediators, both systemically and locally.

KW - Cytokines

KW - Enteral nutrition

KW - Lipopolysaccharide

KW - Liver

KW - Stress proteins

UR - http://www.scopus.com/inward/record.url?scp=63249103692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63249103692&partnerID=8YFLogxK

U2 - 10.1007/s10620-008-0425-5

DO - 10.1007/s10620-008-0425-5

M3 - Article

VL - 54

SP - 767

EP - 773

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -