FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair

Ting Liu, Gargi Ghosal, Jingsong Yuan, Junjie Chen, Jun Huang

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Fanconi anemia (FA) is caused by mutations in 13 Fanc genes and renders cells hypersensitive to DNA interstrand cross-linking (ICL) agents. A central event in the FA pathway is mono-ubiquitylation of the FANCI-FANCD2 (ID) protein complex. Here, we characterize a previously unrecognized nuclease, Fanconi anemia-associated nuclease 1 (FAN1), that promotes ICL repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the ID complex. Thus, the mono-ubiquitylated ID complex recruits the downstream repair protein FAN1 and facilitates the repair of DNA interstrand cross-links.

Original languageEnglish (US)
Pages (from-to)693-696
Number of pages4
JournalScience
Volume329
Issue number5992
DOIs
StatePublished - Aug 6 2010

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Fanconi Anemia
Ubiquitination
Fanconi Anemia Complementation Group D2 Protein
DNA
Fanconi Anemia Complementation Group Proteins
DNA Repair
DNA Damage
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair. / Liu, Ting; Ghosal, Gargi; Yuan, Jingsong; Chen, Junjie; Huang, Jun.

In: Science, Vol. 329, No. 5992, 06.08.2010, p. 693-696.

Research output: Contribution to journalArticle

Liu, Ting ; Ghosal, Gargi ; Yuan, Jingsong ; Chen, Junjie ; Huang, Jun. / FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair. In: Science. 2010 ; Vol. 329, No. 5992. pp. 693-696.
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