F-actin is concentrated in nonrelease domains at frog neuromuscular junctions

Anna Dunaevsky, Elizabeth A. Connor

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

To gain insight into the role of F-actin in the organization of synaptic vesicles at release sites, we examined the synaptic distribution of F-actin by using a unique synaptic preparation of frog target-deprived nerve terminals. In this preparation, imaging of the synaptic site was unobstructed by the muscle fiber cytoskeleton, allowing for the examination of hundreds of synaptic sites in their entirety in whole mounts. At target-deprived synaptic sites F-actin was distributed in a ladder-like pattern and was colocalized with β-fodrin. Surprisingly, F-actin stain, which we localized to the nerve terminal itself, did not overlap a synaptic vesicle marker, suggesting that it was concentrated in nonrelease domains of nerve terminals between clusters of synaptic vesicles. These findings suggest that the majority of the presynaptic F-actin is not involved in tethering synaptic vesicles. Instead, the strategic presynaptic positioning of this cytoskeletal meshwork in nonrelease domains of the nerve terminal suggests alternate functions such as restricting synaptic vesicles to release domains, recycling synaptic vesicles, or stabilizing the nerve terminal.

Original languageEnglish (US)
Pages (from-to)6007-6012
Number of pages6
JournalJournal of Neuroscience
Volume20
Issue number16
StatePublished - Aug 15 2000

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Synaptic Vesicles
Neuromuscular Junction
Anura
Actins
Cytoskeleton
Coloring Agents
Muscles

Keywords

  • Cytoskeleton
  • F-actin
  • Presynaptic
  • Synaptic vesicles
  • Terminal Schwann cell
  • β-Fodrin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

F-actin is concentrated in nonrelease domains at frog neuromuscular junctions. / Dunaevsky, Anna; Connor, Elizabeth A.

In: Journal of Neuroscience, Vol. 20, No. 16, 15.08.2000, p. 6007-6012.

Research output: Contribution to journalArticle

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