Extraction of omega-3-rich oil from Camelina sativa seed using supercritical carbon dioxide

Henok D. Belayneh, Randy L. Wehling, Edgar Cahoon, Ozan N. Ciftci

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Camelina sativa seed is an underutilized oil source; however, it is a potential source of omega-3-rich oil. Supercritical carbon dioxide (SC-CO2) is a desired green solvent for the extraction of omega-3 oils; however, there is no reported study on the SC-CO2 extraction of Camelina seed oil. Therefore, the objective of this study was to evaluate the use of SC-CO2 for the extraction of Camelina seed oil and to compare with traditional extraction methods. Response surface methodology (RSM) based on central composite rotatable design was employed to investigate the extraction conditions: pressure (35-45MPa), temperature (50-70°C), and time (90-250min). A second order polynomial model was developed to predict the oil yield. Increasing pressure and time increased the oil yield, whereas increasing temperature did not have a significant effect. RSM-optimized conditions (45MPa, 70°C and 224.5min) obtained by RSM for the range of variables predicted a yield of 27.0% oil, whereas the actual yield was 25.1±2.0%. Oil yield was further increased to 31.6% at the RSM-optimized conditions by increasing the SC-CO2 extraction time to 510min. Soxhlet (hexane) and cold press methods yielded 35.9% and 29.9% oil, respectively. Extraction method did not have a significant effect on the fatty acid composition and tocopherol content (P >0.05); however, phytosterol content of the cold pressed oil was significantly lower than that of SC-CO2 and Soxhlet (hexane) (P <0.05). Results indicated that SC-CO2 is a promising green solvent for the extraction of Camelina seed oil.

Original languageEnglish (US)
JournalJournal of Supercritical Fluids
Volume104
DOIs
StateAccepted/In press - Apr 16 2015

Fingerprint

Carbon Dioxide
Seed
carbon dioxide
seeds
Carbon dioxide
Oils
oils
Oilseeds
Hexanes
methodology
Hexane
Phytosterols
Tocopherols
tocopherol
Fatty acids
fatty acids
Fatty Acids
Temperature
polynomials
Composite materials

Keywords

  • Camelina sativa
  • Extraction
  • Fatty acid
  • Omega-3
  • Phytosterol
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Extraction of omega-3-rich oil from Camelina sativa seed using supercritical carbon dioxide. / Belayneh, Henok D.; Wehling, Randy L.; Cahoon, Edgar; Ciftci, Ozan N.

In: Journal of Supercritical Fluids, Vol. 104, 16.04.2015.

Research output: Contribution to journalArticle

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abstract = "Camelina sativa seed is an underutilized oil source; however, it is a potential source of omega-3-rich oil. Supercritical carbon dioxide (SC-CO2) is a desired green solvent for the extraction of omega-3 oils; however, there is no reported study on the SC-CO2 extraction of Camelina seed oil. Therefore, the objective of this study was to evaluate the use of SC-CO2 for the extraction of Camelina seed oil and to compare with traditional extraction methods. Response surface methodology (RSM) based on central composite rotatable design was employed to investigate the extraction conditions: pressure (35-45MPa), temperature (50-70°C), and time (90-250min). A second order polynomial model was developed to predict the oil yield. Increasing pressure and time increased the oil yield, whereas increasing temperature did not have a significant effect. RSM-optimized conditions (45MPa, 70°C and 224.5min) obtained by RSM for the range of variables predicted a yield of 27.0{\%} oil, whereas the actual yield was 25.1±2.0{\%}. Oil yield was further increased to 31.6{\%} at the RSM-optimized conditions by increasing the SC-CO2 extraction time to 510min. Soxhlet (hexane) and cold press methods yielded 35.9{\%} and 29.9{\%} oil, respectively. Extraction method did not have a significant effect on the fatty acid composition and tocopherol content (P >0.05); however, phytosterol content of the cold pressed oil was significantly lower than that of SC-CO2 and Soxhlet (hexane) (P <0.05). Results indicated that SC-CO2 is a promising green solvent for the extraction of Camelina seed oil.",
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