Extraction of astaxanthin from engineered Camelina sativa seed using ethanol-modified supercritical carbon dioxide

Liyang Xie, Edgar Cahoon, Yue Zhang, Ozan N. Ciftci

Research output: Contribution to journalArticle

Abstract

Natural astaxanthin, a high-value carotenoid that is currently extracted mainly from marine organisms, was extracted from engineered camelina seed using ethanol-modified supercritical carbon dioxide (SC-CO2) for the first time, and compared with hexane and accelerated solvent extraction using hexane and ethanol. Response surface methodology (RSM) based on central composite rotatable design was employed to investigate the effect of pressure (30–45 MPa), temperature (40–60 °C), and ethanol concentration (10–35%, w/w). RSM-optimized conditions (41.6 MPa, 36.6 °C and 42.0% ethanol concentration) predicted the astaxanthin concentration as 437 μg/g oil, whereas the actual concentration was 421 ± 14 μg/g oil. Astaxanthin concentration in accelerated solvent extracted oil was significantly lower than that in ethanol-modified SC-CO2- and hexane-extracted oils (P < 0.05). Oils extracted with ethanol-modified SC-CO2 had the highest antioxidant activity. Results indicated that ethanol-modified SC-CO2 extraction method can be successfully used as a green method to extract astaxanthin from high oil feedstocks.

LanguageEnglish (US)
Pages171-178
Number of pages8
JournalJournal of Supercritical Fluids
Volume143
DOIs
StatePublished - Jan 1 2019

Fingerprint

Carbon Dioxide
Seed
carbon dioxide
seeds
Carbon dioxide
Ethanol
ethyl alcohol
Oils
oils
Hexanes
Hexane
methodology
carotenoids
solvent extraction
antioxidants
Solvent extraction
Carotenoids
astaxanthine
Antioxidants
organisms

Keywords

  • Astaxanthin
  • Camelina seed
  • Extraction
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Extraction of astaxanthin from engineered Camelina sativa seed using ethanol-modified supercritical carbon dioxide. / Xie, Liyang; Cahoon, Edgar; Zhang, Yue; Ciftci, Ozan N.

In: Journal of Supercritical Fluids, Vol. 143, 01.01.2019, p. 171-178.

Research output: Contribution to journalArticle

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