Extraction and characterization of lipids from the kernels, leaves, and stalks of nine grain sorghum parent lines

K. L. Christiansen, C. L. Weller, Vicki L Schlegel, S. L. Cuppett, Timothy P Carr

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The objectives of this study were to identify and quantify lipid classes extracted from the stalk, leaves, and the whole kernels of nine different sorghum parent lines. All extractions were performed with a Soxtec using H-hexane as the extraction solvent. Analytical methods including high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC) were used to identify and quantify the extracted lipid classes: triacylglycerides (TAG), diglycerides (DG), policosanols (PA), free sterols (FS), phytosterols (PS), free fatty acids (FFA), tocopherols (T), and fatty aldehydes. Overall, extracts from the leaves had higher amounts of soluble matter. Extracts from leaves contained more alcohols and sterols, whereas extracts from the stalks and the whole kernels contained the greatest amount of free fatty acids and fatty aldehydes, respectively. Trace amounts of tocopherol were detected by HPLC in all 27 samples. The lipid profiles of extracts from the whole kernels, leaves, and stalks from the same plant differed. The lipid profiles of extracts from each anatomical part of the sorghum plant differed between the nine parent lines

Original languageEnglish (US)
Pages (from-to)463-470
Number of pages8
JournalCereal Chemistry
Volume84
Issue number5
DOIs
StatePublished - Sep 1 2007

Fingerprint

Sorghum
grain sorghum
Lipids
policosanol
Tocopherols
extracts
High performance liquid chromatography
Sterols
lipids
seeds
Nonesterified Fatty Acids
leaves
tocopherols
aldehydes
sterols
High Pressure Liquid Chromatography
free fatty acids
Phytosterols
Thin layer chromatography
high performance liquid chromatography

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Extraction and characterization of lipids from the kernels, leaves, and stalks of nine grain sorghum parent lines. / Christiansen, K. L.; Weller, C. L.; Schlegel, Vicki L; Cuppett, S. L.; Carr, Timothy P.

In: Cereal Chemistry, Vol. 84, No. 5, 01.09.2007, p. 463-470.

Research output: Contribution to journalArticle

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