Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster

Greg Gibson, Rebecca Riley-Berger, Larry Harshman, Artyom Kopp, Scott Vacha, Sergey Nuzhdin, Marta Wayne

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Assessment of the degree to which gene expression is additive and heritable has important implications for understanding the maintenance of variation, adaptation, phenotypic divergence, and the mapping of genotype onto phenotype. We used whole-genome transcript profiling using Agilent long-oligonucleotide microarrays representing 12,017 genes to demonstrate that gene transcription is pervasively nonadditive in Drosophila melanogaster. Comparison of adults of two isogenic lines and their reciprocal F1 hybrids revealed 5820 genes as significantly different between at least two of the four genotypes in either males or females or across both sexes. Strikingly, while 25% of all genes differ between the two parents, 33% differ between both F1's and the parents, averaged across sexes. However, only 5% of genes show overdominance, suggesting that heterosis for expression is rare.

Original languageEnglish (US)
Pages (from-to)1791-1799
Number of pages9
JournalGenetics
Volume167
Issue number4
DOIs
StatePublished - Aug 1 2004

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Drosophila melanogaster
Gene Expression
Genes
Genotype
Hybrid Vigor
Oligonucleotide Array Sequence Analysis
Maintenance
Genome
Phenotype

ASJC Scopus subject areas

  • Genetics

Cite this

Gibson, G., Riley-Berger, R., Harshman, L., Kopp, A., Vacha, S., Nuzhdin, S., & Wayne, M. (2004). Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster. Genetics, 167(4), 1791-1799. https://doi.org/10.1534/genetics.104.026583

Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster. / Gibson, Greg; Riley-Berger, Rebecca; Harshman, Larry; Kopp, Artyom; Vacha, Scott; Nuzhdin, Sergey; Wayne, Marta.

In: Genetics, Vol. 167, No. 4, 01.08.2004, p. 1791-1799.

Research output: Contribution to journalArticle

Gibson, G, Riley-Berger, R, Harshman, L, Kopp, A, Vacha, S, Nuzhdin, S & Wayne, M 2004, 'Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster', Genetics, vol. 167, no. 4, pp. 1791-1799. https://doi.org/10.1534/genetics.104.026583
Gibson G, Riley-Berger R, Harshman L, Kopp A, Vacha S, Nuzhdin S et al. Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster. Genetics. 2004 Aug 1;167(4):1791-1799. https://doi.org/10.1534/genetics.104.026583
Gibson, Greg ; Riley-Berger, Rebecca ; Harshman, Larry ; Kopp, Artyom ; Vacha, Scott ; Nuzhdin, Sergey ; Wayne, Marta. / Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster. In: Genetics. 2004 ; Vol. 167, No. 4. pp. 1791-1799.
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