Extension of life-span by introduction of telomerase into normal human cells

Andrea G. Bodnar, Michel M Ouellette, Maria Frolkis, Shawn E. Holt, Choy Pik Chiu, Gregg B. Morin, Calvin B. Harley, Jerry W. Shay, Serge Lichtsteiner, Woodring E. Wright

Research output: Contribution to journalArticle

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Abstract

Normal human cells undergo a finite number of cell divisions and ultimately enter a nondividing state called replicative senescence. It has been proposed that telomere shortening is the molecular clock that triggers senescence. To test this hypothesis, two telomerase-negative normal human cell types, retinal pigment epithelial cells and fore-skin fibroblasts, were transfected with vectors encoding the human telomerase catalytic subunit. In contrast to telomerase-negative control clones, which exhibited telomere shortening and senescence, telomerase-expressing clones had elongated telomeres, divided vigorously, and showed reduced staining for β- galactosidase, a biomarker for senescence. Notably, the telomerase-expressing clones have a normal karyotype and have already exceeded their normal life- span by at least 20 doublings, thus establishing a causal relationship between telomere shortening and in vitro cellular senescence. The ability to maintain normal human cells in a phenotypically youthful state could have important applications in research and medicine.

Original languageEnglish (US)
Pages (from-to)349-352
Number of pages4
JournalScience
Volume279
Issue number5349
DOIs
StatePublished - Jan 16 1998
Externally publishedYes

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Telomerase
Life Expectancy
Telomere Shortening
Clone Cells
Cell Aging
Galactosidases
Retinal Pigments
Telomere
Karyotype
Cell Division
Fibroblasts
Biomarkers
Epithelial Cells
Medicine
Staining and Labeling
Skin
Research

ASJC Scopus subject areas

  • General

Cite this

Bodnar, A. G., Ouellette, M. M., Frolkis, M., Holt, S. E., Chiu, C. P., Morin, G. B., ... Wright, W. E. (1998). Extension of life-span by introduction of telomerase into normal human cells. Science, 279(5349), 349-352. https://doi.org/10.1126/science.279.5349.349

Extension of life-span by introduction of telomerase into normal human cells. / Bodnar, Andrea G.; Ouellette, Michel M; Frolkis, Maria; Holt, Shawn E.; Chiu, Choy Pik; Morin, Gregg B.; Harley, Calvin B.; Shay, Jerry W.; Lichtsteiner, Serge; Wright, Woodring E.

In: Science, Vol. 279, No. 5349, 16.01.1998, p. 349-352.

Research output: Contribution to journalArticle

Bodnar, AG, Ouellette, MM, Frolkis, M, Holt, SE, Chiu, CP, Morin, GB, Harley, CB, Shay, JW, Lichtsteiner, S & Wright, WE 1998, 'Extension of life-span by introduction of telomerase into normal human cells' Science, vol. 279, no. 5349, pp. 349-352. https://doi.org/10.1126/science.279.5349.349
Bodnar, Andrea G. ; Ouellette, Michel M ; Frolkis, Maria ; Holt, Shawn E. ; Chiu, Choy Pik ; Morin, Gregg B. ; Harley, Calvin B. ; Shay, Jerry W. ; Lichtsteiner, Serge ; Wright, Woodring E. / Extension of life-span by introduction of telomerase into normal human cells. In: Science. 1998 ; Vol. 279, No. 5349. pp. 349-352.
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