Expression of transforming growth factor-β3 by embryonal carcinoma cells, parietal endoderm-like cells and early mouse embryos

Wendy J. Campbell, David Lee Kelly, A Angie Rizzino

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15 Citations (Scopus)

Abstract

Utilizing the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have examined the expression of transforming growth factor-beta 3 (TGF-β3) by embryonal carcinoma (EC) cells, EC-derived differentiated cells and early mammalian embryos. Using a TGF-β bioassay, we determined that PYS-2 cells express considerable TGF-β activity that cannot be completely neutralized by antibodies specific for TGF-β1 and TGF-β2. We also have determined that PYS-2 cells, as well as F9 EC cells and their differentiated cells, express transcripts for TGF-β3. In addition, we have determined that blastocysts, cultured for three days in serum-containing medium, express TGF-β3 transcripts. Thus, our data suggest that expression of TGF-β3 is initiated during early stages of mammalian development.

Original languageEnglish (US)
Pages (from-to)1181-1185
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume26
Issue number12
DOIs
StatePublished - Dec 1 1990

Fingerprint

Embryonal Carcinoma Stem Cells
Endoderm
Transforming Growth Factors
Transforming Growth Factor beta
Embryonic Structures
Cells
Mammalian Embryo
Embryonal Carcinoma
Bioassay
Polymerase chain reaction
Blastocyst
Transcription
Biological Assay
Reverse Transcription
Polymerase Chain Reaction
Antibodies
Serum

Keywords

  • embryonal carcinoma cells
  • mammalian embryogenesis
  • polymerase chain reaction
  • transforming growth factor type-beta

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "Utilizing the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have examined the expression of transforming growth factor-beta 3 (TGF-β3) by embryonal carcinoma (EC) cells, EC-derived differentiated cells and early mammalian embryos. Using a TGF-β bioassay, we determined that PYS-2 cells express considerable TGF-β activity that cannot be completely neutralized by antibodies specific for TGF-β1 and TGF-β2. We also have determined that PYS-2 cells, as well as F9 EC cells and their differentiated cells, express transcripts for TGF-β3. In addition, we have determined that blastocysts, cultured for three days in serum-containing medium, express TGF-β3 transcripts. Thus, our data suggest that expression of TGF-β3 is initiated during early stages of mammalian development.",
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AU - Rizzino, A Angie

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