Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines

Marie E. Sutherlin, Isao Nishimori, Thomas Caffrey, Eric P. Bennett, Helle Hassan, Ulla Mandel, David Mack, Takeshi Iwamura, Henrik Clausen, Michael A Hollingsworth

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Abstract

The levels of mRNA expression of three UDP-N-acetyl-α-D- galactosamine:polypeptide GalNAc N-acetylgalactosaminyltransferases (GalNAc- transferases) were quantified for human adenocarcinoma cell lines from pancreas, colon, stomach, and breast. Two of the GalNAc-transferases, GalNAc- T1 and GalNAc-T2, were expressed constitutively and at low levels in most or all cell lines examined. A third GalNAc-transferase, GalNAc-T3, was differentially expressed. Well-differentiated adenocarcinoma cell lines expressed high levels and moderately differentiated cell lines expressed lower levels of GalNAc-T3. Cell lines classified as poorly differentiated failed to express GalNAc-T3 mRNA at levels that could be detected by Northern blot analysis. Differential expression of the GalNAc-T3 protein was confirmed in these cell lines by Western blotting. We propose that glycosylation in tumor cell lines may be regulated in part by differential expression of GalNAc-transferases, and we suggest that GalNAc-T3 gene expression may be a molecular indicator of differentiated adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)4744-4748
Number of pages5
JournalCancer Research
Volume57
Issue number21
StatePublished - Nov 1 1997

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Acetylgalactosamine
Uridine Diphosphate
Adenocarcinoma
Cell Line
Peptides
polypeptide N-acetylgalactosaminyltransferase
Messenger RNA
Tumor Cell Line
Glycosylation
Northern Blotting
Pancreas
Stomach
Colon
Breast
Western Blotting

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines. / Sutherlin, Marie E.; Nishimori, Isao; Caffrey, Thomas; Bennett, Eric P.; Hassan, Helle; Mandel, Ulla; Mack, David; Iwamura, Takeshi; Clausen, Henrik; Hollingsworth, Michael A.

In: Cancer Research, Vol. 57, No. 21, 01.11.1997, p. 4744-4748.

Research output: Contribution to journalArticle

Sutherlin, ME, Nishimori, I, Caffrey, T, Bennett, EP, Hassan, H, Mandel, U, Mack, D, Iwamura, T, Clausen, H & Hollingsworth, MA 1997, 'Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines', Cancer Research, vol. 57, no. 21, pp. 4744-4748.
Sutherlin ME, Nishimori I, Caffrey T, Bennett EP, Hassan H, Mandel U et al. Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines. Cancer Research. 1997 Nov 1;57(21):4744-4748.
Sutherlin, Marie E. ; Nishimori, Isao ; Caffrey, Thomas ; Bennett, Eric P. ; Hassan, Helle ; Mandel, Ulla ; Mack, David ; Iwamura, Takeshi ; Clausen, Henrik ; Hollingsworth, Michael A. / Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines. In: Cancer Research. 1997 ; Vol. 57, No. 21. pp. 4744-4748.
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