Expression of the POTE gene family in human ovarian cancer

Carter J. Barger, Wa Zhang, Ashok Sharma, Linda Chee, Smitha R. James, Christina N. Kufel, Austin Miller, Jane L Meza, Ronny Drapkin, Kunle Odunsi, David L Klinkebiel, Adam R Karpf

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3 Citations (Scopus)

Abstract

The POTE family includes 14 genes in three phylogenetic groups. We determined POTE mRNA expression in normal tissues, epithelial ovarian and high-grade serous ovarian cancer (EOC, HGSC), and pan-cancer, and determined the relationship of POTE expression to ovarian cancer clinicopathology. Groups 1 & 2 POTEs showed testis-specific expression in normal tissues, consistent with assignment as cancer-testis antigens (CTAs), while Group 3 POTEs were expressed in several normal tissues, indicating they are not CTAs. Pan-POTE and individual POTEs showed significantly elevated expression in EOC and HGSC compared to normal controls. Pan-POTE correlated with increased stage, grade, and the HGSC subtype. Select individual POTEs showed increased expression in recurrent HGSC, and POTEE specifically associated with reduced HGSC OS. Consistent with tumors, EOC cell lines had significantly elevated Pan-POTE compared to OSE and FTE cells. Notably, Group 1 & 2 POTEs (POTEs A/B/B2/C/D), Group 3 POTE-actin genes (POTEs E/F/I/J/KP), and other Group 3 POTEs (POTEs G/H/M) show within-group correlated expression, and pan-cancer analyses of tumors and cell lines confirmed this relationship. Based on their restricted expression in normal tissues and increased expression and association with poor prognosis in ovarian cancer, POTEs are potential oncogenes and therapeutic targets in this malignancy.

Original languageEnglish (US)
Article number17136
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Ovarian Neoplasms
Testicular Neoplasms
Tumor Cell Line
Gene Expression
Antigens
Neoplasms
Oncogenes
Genes
Testis
Actins
Epithelium
Messenger RNA
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Barger, C. J., Zhang, W., Sharma, A., Chee, L., James, S. R., Kufel, C. N., ... Karpf, A. R. (2018). Expression of the POTE gene family in human ovarian cancer. Scientific reports, 8(1), [17136]. https://doi.org/10.1038/s41598-018-35567-1

Expression of the POTE gene family in human ovarian cancer. / Barger, Carter J.; Zhang, Wa; Sharma, Ashok; Chee, Linda; James, Smitha R.; Kufel, Christina N.; Miller, Austin; Meza, Jane L; Drapkin, Ronny; Odunsi, Kunle; Klinkebiel, David L; Karpf, Adam R.

In: Scientific reports, Vol. 8, No. 1, 17136, 01.12.2018.

Research output: Contribution to journalArticle

Barger, CJ, Zhang, W, Sharma, A, Chee, L, James, SR, Kufel, CN, Miller, A, Meza, JL, Drapkin, R, Odunsi, K, Klinkebiel, DL & Karpf, AR 2018, 'Expression of the POTE gene family in human ovarian cancer', Scientific reports, vol. 8, no. 1, 17136. https://doi.org/10.1038/s41598-018-35567-1
Barger CJ, Zhang W, Sharma A, Chee L, James SR, Kufel CN et al. Expression of the POTE gene family in human ovarian cancer. Scientific reports. 2018 Dec 1;8(1). 17136. https://doi.org/10.1038/s41598-018-35567-1
Barger, Carter J. ; Zhang, Wa ; Sharma, Ashok ; Chee, Linda ; James, Smitha R. ; Kufel, Christina N. ; Miller, Austin ; Meza, Jane L ; Drapkin, Ronny ; Odunsi, Kunle ; Klinkebiel, David L ; Karpf, Adam R. / Expression of the POTE gene family in human ovarian cancer. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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