Expression of the JE/MCP-1 gene suppresses metastatic potential in murine colon carcinoma cells

Suyun Huang, Rakesh K. Singh, Keping Xie, Mordechai Gutman, Karen K. Berry, Corazon D. Bucana, Isaiah J. Fidler, Menashe Bar-Eli

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine whether the expression of the JE/MCP-1 gene encoding for the monocyte chemottractant protein, MCP-1 (also known as monocyte chemotactic and activating factor MCAF, TDCF, and SMC-CF) can influence the metastatic properties of tumor cells. The highly metastatic murine colon carcinoma CT-26 cells, syngeneic to BALB/c mice that do not produce endogenous JE/MCP-1 protein, were transfected with a BCMGS-Neo expression vector (control) or a vector containing full-length JE cDNA. CT-26 parental cells, CT-26 Neo, and CT-26 JE/MCP-1-positive cells were injected into syngeneic or nude mice. The CT-26 JE/MCP-1-positive cells produced significantly fewer lung metastases. The decrease in incidence of metastasis was not due to the inability of the transfected cells to arrest in the lung vasculature or to differences in cell cycle time. CT-26 cells producing JE/MCP-1 were highly susceptible to lysis by syngeneic macrophages treated with subthreshold concentrations of lipopolysaccharide. In addition, culture supernatants of JE/MCP-1-expressing cells plus lipopolysaccharide synergistically activated tumoricidal properties in syngeneic macrophages. This activity was blocked by anti-JE/MCP-1 antibodies, indicating the involvement of the JE/MCP-1 molecule in this process. Moreover, purified JE/MCP-1 added to lipopolysaccharide-containing medium resulted in significant activation of macrophages against parental CT-26 cells. These data suggest that, in addition to its chemotactic properties, JE/MCP-1 can synergize with bacterial endotoxins to activate macrophages to become tumoricidal and, hence, could suppress metastasis.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalCancer Immunology Immunotherapy
Volume39
Issue number4
DOIs
StatePublished - Jul 1 1994

Fingerprint

Colon
Carcinoma
Genes
Lipopolysaccharides
Macrophages
Neoplasm Metastasis
Lung
Macrophage Activation
Chemokine CCL2
Nude Mice
Endotoxins
Monocytes
Cell Cycle
Proteins
Complementary DNA
Antibodies
Incidence
Neoplasms

Keywords

  • Colon carcinoma
  • Cytokines
  • Metastasis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cite this

Expression of the JE/MCP-1 gene suppresses metastatic potential in murine colon carcinoma cells. / Huang, Suyun; Singh, Rakesh K.; Xie, Keping; Gutman, Mordechai; Berry, Karen K.; Bucana, Corazon D.; Fidler, Isaiah J.; Bar-Eli, Menashe.

In: Cancer Immunology Immunotherapy, Vol. 39, No. 4, 01.07.1994, p. 231-238.

Research output: Contribution to journalArticle

Huang, S, Singh, RK, Xie, K, Gutman, M, Berry, KK, Bucana, CD, Fidler, IJ & Bar-Eli, M 1994, 'Expression of the JE/MCP-1 gene suppresses metastatic potential in murine colon carcinoma cells', Cancer Immunology Immunotherapy, vol. 39, no. 4, pp. 231-238. https://doi.org/10.1007/BF01525986
Huang, Suyun ; Singh, Rakesh K. ; Xie, Keping ; Gutman, Mordechai ; Berry, Karen K. ; Bucana, Corazon D. ; Fidler, Isaiah J. ; Bar-Eli, Menashe. / Expression of the JE/MCP-1 gene suppresses metastatic potential in murine colon carcinoma cells. In: Cancer Immunology Immunotherapy. 1994 ; Vol. 39, No. 4. pp. 231-238.
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