Expression of the human mucin gene, Muc 1, in normal tissues and metastatic pancreatic tumors

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9 Citations (Scopus)

Abstract

Expression of Muc 1 mRNA was evaluated in normal and metastatic-tumor tissues obtained from a patient who died from extensive metastasis of a well-diffferentiated pancreatic adenocarcinoma. Northern blot analysis of total RNA from these tissues revealed that Muc 1 mRNA was detectable and unaltered in size in all metastatic tumor samples tested, including those from lymph node, liver, colon, and stomach. Southern blot analysis indicated that two forms of Muc 1 gene alleles were present in all normal and metastatic tissuess of this patient with no evidence for gross rearrangement, deletion, or duplication.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalInternational Journal of Pancreatology
Volume10
Issue number3-4
DOIs
StatePublished - Nov 1 1991

Fingerprint

Mucins
Messenger RNA
Southern Blotting
Northern Blotting
Genes
Neoplasms
Stomach
Colon
Adenocarcinoma
Lymph Nodes
Alleles
RNA
Neoplasm Metastasis
Liver

Keywords

  • Muc 1
  • Northern blot
  • Southern blot
  • mucin expression, adenocarcinoma
  • tandem-repeat length

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

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abstract = "Expression of Muc 1 mRNA was evaluated in normal and metastatic-tumor tissues obtained from a patient who died from extensive metastasis of a well-diffferentiated pancreatic adenocarcinoma. Northern blot analysis of total RNA from these tissues revealed that Muc 1 mRNA was detectable and unaltered in size in all metastatic tumor samples tested, including those from lymph node, liver, colon, and stomach. Southern blot analysis indicated that two forms of Muc 1 gene alleles were present in all normal and metastatic tissuess of this patient with no evidence for gross rearrangement, deletion, or duplication.",
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T1 - Expression of the human mucin gene, Muc 1, in normal tissues and metastatic pancreatic tumors

AU - Batra, Surinder K.

AU - Hollingsworth, Michael A.

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N2 - Expression of Muc 1 mRNA was evaluated in normal and metastatic-tumor tissues obtained from a patient who died from extensive metastasis of a well-diffferentiated pancreatic adenocarcinoma. Northern blot analysis of total RNA from these tissues revealed that Muc 1 mRNA was detectable and unaltered in size in all metastatic tumor samples tested, including those from lymph node, liver, colon, and stomach. Southern blot analysis indicated that two forms of Muc 1 gene alleles were present in all normal and metastatic tissuess of this patient with no evidence for gross rearrangement, deletion, or duplication.

AB - Expression of Muc 1 mRNA was evaluated in normal and metastatic-tumor tissues obtained from a patient who died from extensive metastasis of a well-diffferentiated pancreatic adenocarcinoma. Northern blot analysis of total RNA from these tissues revealed that Muc 1 mRNA was detectable and unaltered in size in all metastatic tumor samples tested, including those from lymph node, liver, colon, and stomach. Southern blot analysis indicated that two forms of Muc 1 gene alleles were present in all normal and metastatic tissuess of this patient with no evidence for gross rearrangement, deletion, or duplication.

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KW - Southern blot

KW - mucin expression, adenocarcinoma

KW - tandem-repeat length

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