Expression of the human MUC1 mucin cDNA in a hamster pancreatic tumor cell line HP-1

Surinder Kumar Batra, Richard S. Metzgar, Andrew J. Worlock, Michael A Hollingsworth

Research output: Contribution to journalArticle

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Abstract

A full length cDNA for the human mucin gene, MUC1, under the control of human β actin promoter, was transfected into a carcinogen induced hamster pancreatic ductal tumor cell line, HP-1. Transfectants were selected by resistance to geneticin. Integration of the foreign human MUC1 cDNA occurred at multiple sites in the genome of HP-1. Northern blot analysis showed MUC1 expression in cells transfected with MUC1 cDNA placed in the correct orientation, but not in control cells (HP-1 cells transfected with vector alone, or with the MUC1 cDNA placed in the antisense orientation). Western blot analysis using monoclonal antibody HMFG-2, which is reactive with the MUC1 protein, showed results consistent with the Northern blot data. Positive immunoperoxidase staining using HMFG-2 was seen in HP-1 cells transfected with MUC1 cDNA but not with untransfected or HP-1 control cells. The integration of human MUC1 mucin gene in HP-1 cells caused no significant change in the growth rate of HP-1 cells in vitro, but resulted in an enhanced growth rate for xenografts of MUC1 transfected HP-1 cells grown in nude mice.

Original languageEnglish (US)
Pages (from-to)271-283
Number of pages13
JournalInternational Journal of Pancreatology
Volume12
Issue number3
DOIs
StatePublished - Dec 1 1992

Fingerprint

Mucins
Tumor Cell Line
Cricetinae
Complementary DNA
Northern Blotting
Growth
Heterografts
Nude Mice
Carcinogens
Genes
Actins
Western Blotting
Monoclonal Antibodies
Genome
Staining and Labeling

Keywords

  • MUC1 expression
  • Pancreatic cancer
  • mucin

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

Expression of the human MUC1 mucin cDNA in a hamster pancreatic tumor cell line HP-1. / Batra, Surinder Kumar; Metzgar, Richard S.; Worlock, Andrew J.; Hollingsworth, Michael A.

In: International Journal of Pancreatology, Vol. 12, No. 3, 01.12.1992, p. 271-283.

Research output: Contribution to journalArticle

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