Abstract

Tumor associated glycoprotein-72 (TAG-72), a pancarcinoma antigen, was initially identified in cancer tissues by its immunoreactivity to a monoclonal antibody B72.3. In this study, we have analyzed the expression and localization profiles of TAG-72 in ovarian cancer tissue samples of different stages and histological subtypes by immunohistochemistry using a second generation high affinity monoclonal antibody CC49. We have also studied the expression of TAG-72 in ovarian cancer cell lines by confocal microscopy and immunoblot analyses. A correlation between TAG-72 expression and localization with patients' prognosis was also analyzed using Kaplan-Meier analysis. Eighty eight percent of the ovarian cancer tissue samples (n = 43) showed immunoreactivity with CC49 antibody. The expression of TAG-72 in advanced stage cancer tissues (mean composite score = 3.7) was significantly higher (p = 0.035) compared to the early stage tumors (mean composite score = 2.3). However, no significant correlation of TAG-72 was observed with histological tumor types. A marginal correlation of TAG-72 staining with patients' survival was observed. Interestingly, the membrane localization of TAG-72 in tumors was significantly (p = 0.0082) associated to the poor clinical outcome, while cytoplasmic staining was correlated significantly to a better prognosis (p = 0.0051). Immunoblot analysis demonstrated the expression of TAG-72 in three ovarian cancer cell lines (OVCAR3, SB247 and COV362.4). In conclusion, the tumor-specific expression of TAG-72 and its association with disease stage indicate its potential as a marker for effective disease management and targeted cancer therapy.

Original languageEnglish (US)
Pages (from-to)247-257
Number of pages11
JournalCancer Letters
Volume251
Issue number2
DOIs
StatePublished - Jun 28 2007

Fingerprint

Ovarian Neoplasms
Neoplasms
Monoclonal Antibodies
tumor-associated antigen 72
Staining and Labeling
Cell Line
Kaplan-Meier Estimate
Disease Management
Confocal Microscopy
Immunohistochemistry
Antigens
Membranes
Survival

Keywords

  • Diagnosis
  • Ovarian cancer
  • Prognosis
  • TAG-72
  • Therapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Expression of TAG-72 in ovarian cancer and its correlation with tumor stage and patient prognosis. / Palanimuthu Ponnusamy, Moorthy; Venkatraman, Ganesh; Singh, Ajay P.; Chauhan, Subhash C.; Johansson, Sonny L.; Jain, Maneesh; Smith, Lynette M; Davis, John S; Remmenga, Steven W; Batra, Surinder Kumar.

In: Cancer Letters, Vol. 251, No. 2, 28.06.2007, p. 247-257.

Research output: Contribution to journalArticle

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abstract = "Tumor associated glycoprotein-72 (TAG-72), a pancarcinoma antigen, was initially identified in cancer tissues by its immunoreactivity to a monoclonal antibody B72.3. In this study, we have analyzed the expression and localization profiles of TAG-72 in ovarian cancer tissue samples of different stages and histological subtypes by immunohistochemistry using a second generation high affinity monoclonal antibody CC49. We have also studied the expression of TAG-72 in ovarian cancer cell lines by confocal microscopy and immunoblot analyses. A correlation between TAG-72 expression and localization with patients' prognosis was also analyzed using Kaplan-Meier analysis. Eighty eight percent of the ovarian cancer tissue samples (n = 43) showed immunoreactivity with CC49 antibody. The expression of TAG-72 in advanced stage cancer tissues (mean composite score = 3.7) was significantly higher (p = 0.035) compared to the early stage tumors (mean composite score = 2.3). However, no significant correlation of TAG-72 was observed with histological tumor types. A marginal correlation of TAG-72 staining with patients' survival was observed. Interestingly, the membrane localization of TAG-72 in tumors was significantly (p = 0.0082) associated to the poor clinical outcome, while cytoplasmic staining was correlated significantly to a better prognosis (p = 0.0051). Immunoblot analysis demonstrated the expression of TAG-72 in three ovarian cancer cell lines (OVCAR3, SB247 and COV362.4). In conclusion, the tumor-specific expression of TAG-72 and its association with disease stage indicate its potential as a marker for effective disease management and targeted cancer therapy.",
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T1 - Expression of TAG-72 in ovarian cancer and its correlation with tumor stage and patient prognosis

AU - Palanimuthu Ponnusamy, Moorthy

AU - Venkatraman, Ganesh

AU - Singh, Ajay P.

AU - Chauhan, Subhash C.

AU - Johansson, Sonny L.

AU - Jain, Maneesh

AU - Smith, Lynette M

AU - Davis, John S

AU - Remmenga, Steven W

AU - Batra, Surinder Kumar

PY - 2007/6/28

Y1 - 2007/6/28

N2 - Tumor associated glycoprotein-72 (TAG-72), a pancarcinoma antigen, was initially identified in cancer tissues by its immunoreactivity to a monoclonal antibody B72.3. In this study, we have analyzed the expression and localization profiles of TAG-72 in ovarian cancer tissue samples of different stages and histological subtypes by immunohistochemistry using a second generation high affinity monoclonal antibody CC49. We have also studied the expression of TAG-72 in ovarian cancer cell lines by confocal microscopy and immunoblot analyses. A correlation between TAG-72 expression and localization with patients' prognosis was also analyzed using Kaplan-Meier analysis. Eighty eight percent of the ovarian cancer tissue samples (n = 43) showed immunoreactivity with CC49 antibody. The expression of TAG-72 in advanced stage cancer tissues (mean composite score = 3.7) was significantly higher (p = 0.035) compared to the early stage tumors (mean composite score = 2.3). However, no significant correlation of TAG-72 was observed with histological tumor types. A marginal correlation of TAG-72 staining with patients' survival was observed. Interestingly, the membrane localization of TAG-72 in tumors was significantly (p = 0.0082) associated to the poor clinical outcome, while cytoplasmic staining was correlated significantly to a better prognosis (p = 0.0051). Immunoblot analysis demonstrated the expression of TAG-72 in three ovarian cancer cell lines (OVCAR3, SB247 and COV362.4). In conclusion, the tumor-specific expression of TAG-72 and its association with disease stage indicate its potential as a marker for effective disease management and targeted cancer therapy.

AB - Tumor associated glycoprotein-72 (TAG-72), a pancarcinoma antigen, was initially identified in cancer tissues by its immunoreactivity to a monoclonal antibody B72.3. In this study, we have analyzed the expression and localization profiles of TAG-72 in ovarian cancer tissue samples of different stages and histological subtypes by immunohistochemistry using a second generation high affinity monoclonal antibody CC49. We have also studied the expression of TAG-72 in ovarian cancer cell lines by confocal microscopy and immunoblot analyses. A correlation between TAG-72 expression and localization with patients' prognosis was also analyzed using Kaplan-Meier analysis. Eighty eight percent of the ovarian cancer tissue samples (n = 43) showed immunoreactivity with CC49 antibody. The expression of TAG-72 in advanced stage cancer tissues (mean composite score = 3.7) was significantly higher (p = 0.035) compared to the early stage tumors (mean composite score = 2.3). However, no significant correlation of TAG-72 was observed with histological tumor types. A marginal correlation of TAG-72 staining with patients' survival was observed. Interestingly, the membrane localization of TAG-72 in tumors was significantly (p = 0.0082) associated to the poor clinical outcome, while cytoplasmic staining was correlated significantly to a better prognosis (p = 0.0051). Immunoblot analysis demonstrated the expression of TAG-72 in three ovarian cancer cell lines (OVCAR3, SB247 and COV362.4). In conclusion, the tumor-specific expression of TAG-72 and its association with disease stage indicate its potential as a marker for effective disease management and targeted cancer therapy.

KW - Diagnosis

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KW - Therapy

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