Expression of reciprocal fusion transcripts of the HMGIC and LPP genes in parosteal lipoma

Marleen M R Petit, Sarah Swarts, Julia A. Bridge, Wim J M Van De Ven

Research output: Contribution to journalArticle

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Abstract

Parosteal lipomas are rare benign neoplasms of adipose tissue that exhibit a contiguous relationship with the periosteum. These lipomas of the bone share some histopathologic features with their commonly occurring soft tissue counterparts. The latter are well-characterized cytogenetically, primarily by rearrangements involving chromosome region 12q13-q15. In particular, translocations involving 12q13-q15 are prominent, with chromosomal region 3q27-q28 as the most frequent translocation partner. Recently, we established that the genes HMGIC at 12q15 and LPP at 3q27-28 are affected by the 3;12-translocation and demonstrated that, as a direct result, HMGIC/LPP and LPP/HMGIC fusion transcripts are expressed in soft tissue lipomas. In this study, cytogenetic and molecular analyses revealed similar findings in a parosteal lipoma. Specifically, a t(3;12)(q28;q14) was detected cytogenetically in a parosteal lipoma from a 51-year-old female and subsequently confirmed by FISH utilizing a chromosome 3 breakpoint spanning YAC probe and chromosome 12 breakpoint flanking cosmid probes. RT-PCR analysis showed expression of HMGIC/LPP and LPP/HMGIC fusion transcripts in this parosteal lipoma; nucleotide sequence analysis revealed that these transcripts are identical to those expressed in soft tissue lipomas characterized by a 3;12-translocation. These findings lend further support to a common histopathogenesis between lipomas of soft tissue and parosteal origin.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalCancer genetics and cytogenetics
Volume106
Issue number1
DOIs
StatePublished - Oct 1 1998

Fingerprint

Lipoma
Genes
Chromosome Breakpoints
Yeast Artificial Chromosomes
Chromosomes, Human, Pair 12
Periosteum
Cosmids
Chromosomes, Human, Pair 3
Cytogenetic Analysis
Sequence Analysis
Adipose Tissue
Chromosomes
Bone and Bones
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Expression of reciprocal fusion transcripts of the HMGIC and LPP genes in parosteal lipoma. / Petit, Marleen M R; Swarts, Sarah; Bridge, Julia A.; Van De Ven, Wim J M.

In: Cancer genetics and cytogenetics, Vol. 106, No. 1, 01.10.1998, p. 18-23.

Research output: Contribution to journalArticle

Petit, Marleen M R ; Swarts, Sarah ; Bridge, Julia A. ; Van De Ven, Wim J M. / Expression of reciprocal fusion transcripts of the HMGIC and LPP genes in parosteal lipoma. In: Cancer genetics and cytogenetics. 1998 ; Vol. 106, No. 1. pp. 18-23.
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