Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells: Enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke

Anthony A. Floreani, Art J. Heires, Lisbeth A. Welniak, Amanda Miller-Lindholm, Laurel Clark-Pierce, Stephen I. Rennard, Edward L. Morgan, Sam D. Sanderson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Results are presented that demonstrate a heightened responsiveness of human bronchial epithelial cells (HBECs) toward the complement-derived anaphylatoxin C5a when these cells are exposed to cigarette smoke. This C5a response is possible because we show at both the protein and mRNA levels that HBECs constitutively express receptors for C5a (C5aR, CD88). Control (untreated) HBECs responded to C5a (50 nM) by releasing the proinflammatory cytokine IL-8 at low but significant levels. However, exposure of HBECs to 5% cigarette smoke extract (CSE) for at least 15 min resulted in an increase in the ability of an anti-human C5aR Ab to bind to the cell surface. CSE- treated HBECs responded in a dose-dependent fashion to human recombinant C5a and to a conformationally biased decapeptide agonist of C5a (YSFKPMPLaR) by releasing IL-8. The levels of IL-8 released in response to C5a were significantly greater in CSE-treated HBECs than in control HBECs. Moreover, this C5a-mediated release of IL-8 from CSE-treated HBECs was significantly reduced in the presence of the anti-human C5aR Ab. These results indicate that HBECs constitutively express C5aRs and that exposure to environmental irritants such as cigarette smoke modulates the expression and responsiveness of these C5aRs toward the C5a-mediated release of IL-8.

Original languageEnglish (US)
Pages (from-to)5073-5081
Number of pages9
JournalJournal of Immunology
Volume160
Issue number10
StatePublished - May 15 1998

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Anaphylatoxin C5a Receptor
Interleukin-8
Smoke
Tobacco Products
Epithelial Cells
Anaphylatoxins
Irritants
Environmental Exposure

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Floreani, A. A., Heires, A. J., Welniak, L. A., Miller-Lindholm, A., Clark-Pierce, L., Rennard, S. I., ... Sanderson, S. D. (1998). Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells: Enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke. Journal of Immunology, 160(10), 5073-5081.

Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells : Enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke. / Floreani, Anthony A.; Heires, Art J.; Welniak, Lisbeth A.; Miller-Lindholm, Amanda; Clark-Pierce, Laurel; Rennard, Stephen I.; Morgan, Edward L.; Sanderson, Sam D.

In: Journal of Immunology, Vol. 160, No. 10, 15.05.1998, p. 5073-5081.

Research output: Contribution to journalArticle

Floreani, AA, Heires, AJ, Welniak, LA, Miller-Lindholm, A, Clark-Pierce, L, Rennard, SI, Morgan, EL & Sanderson, SD 1998, 'Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells: Enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke', Journal of Immunology, vol. 160, no. 10, pp. 5073-5081.
Floreani, Anthony A. ; Heires, Art J. ; Welniak, Lisbeth A. ; Miller-Lindholm, Amanda ; Clark-Pierce, Laurel ; Rennard, Stephen I. ; Morgan, Edward L. ; Sanderson, Sam D. / Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells : Enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke. In: Journal of Immunology. 1998 ; Vol. 160, No. 10. pp. 5073-5081.
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abstract = "Results are presented that demonstrate a heightened responsiveness of human bronchial epithelial cells (HBECs) toward the complement-derived anaphylatoxin C5a when these cells are exposed to cigarette smoke. This C5a response is possible because we show at both the protein and mRNA levels that HBECs constitutively express receptors for C5a (C5aR, CD88). Control (untreated) HBECs responded to C5a (50 nM) by releasing the proinflammatory cytokine IL-8 at low but significant levels. However, exposure of HBECs to 5{\%} cigarette smoke extract (CSE) for at least 15 min resulted in an increase in the ability of an anti-human C5aR Ab to bind to the cell surface. CSE- treated HBECs responded in a dose-dependent fashion to human recombinant C5a and to a conformationally biased decapeptide agonist of C5a (YSFKPMPLaR) by releasing IL-8. The levels of IL-8 released in response to C5a were significantly greater in CSE-treated HBECs than in control HBECs. Moreover, this C5a-mediated release of IL-8 from CSE-treated HBECs was significantly reduced in the presence of the anti-human C5aR Ab. These results indicate that HBECs constitutively express C5aRs and that exposure to environmental irritants such as cigarette smoke modulates the expression and responsiveness of these C5aRs toward the C5a-mediated release of IL-8.",
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