Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines

Xuanmin He, Lawrence E. Ostrowski, Henry S. Friedman, Carol J. Wikstrand, Sandra H. Bigner, Ahmed Rasheed, Surinder Kumar Batra, Darell D. Signer

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Six well characterized human medulloblastoma cell lines (D283 Med, Daoy, D341 Med, D384 Med, D425 Med, and D458 Med) were examined for the expression of O6-methylguanine-DNA methyltransferase (MGMT) by activity and Western and Northern blot analysis. High levels of MGMT activity were present in D283 Med, Daoy, D341 Med, and D384 Med (136, 0.80, 1.68, and 1.62 pmol/mg of protein, respectively), but negligible MGMT activity was detected in D425 Med and D458 Med (0.06 and 0.05 pmol/mg of protein, respectively), which were derived separately at different times from the same patient. The presence of MGMT protein and its transcript was demonstrated in D283 Med, Daoy, D341 Med, and D384 Med, but both the protein and the mRNA were undetectable in D425 Med and D458 Med. Nevertheless, all six cell lines contained an apparently unaltered MGMT gene, as determined by Southern blot analysis. The absence of MGMT activity in D425 Med and D458 Med is likely due to the absence of the protein, resulting from a lack of transcription of the MGMT gene. The varying levels of expression of MGMT in medulloblastoma cells found in this study should provide a molecular basis for drug design and selection in chemotherapy of this tumor.

Original languageEnglish (US)
Pages (from-to)1144-1148
Number of pages5
JournalCancer Research
Volume52
Issue number5
StatePublished - Jan 1 1992

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Medulloblastoma
Methyltransferases
Cell Line
DNA
Proteins
Protein Methyltransferases
O-(6)-methylguanine
Drug Design
Southern Blotting
Northern Blotting
Genes
Western Blotting
Drug Therapy
Messenger RNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

He, X., Ostrowski, L. E., Friedman, H. S., Wikstrand, C. J., Bigner, S. H., Rasheed, A., ... Signer, D. D. (1992). Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines. Cancer Research, 52(5), 1144-1148.

Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines. / He, Xuanmin; Ostrowski, Lawrence E.; Friedman, Henry S.; Wikstrand, Carol J.; Bigner, Sandra H.; Rasheed, Ahmed; Batra, Surinder Kumar; Signer, Darell D.

In: Cancer Research, Vol. 52, No. 5, 01.01.1992, p. 1144-1148.

Research output: Contribution to journalArticle

He, X, Ostrowski, LE, Friedman, HS, Wikstrand, CJ, Bigner, SH, Rasheed, A, Batra, SK & Signer, DD 1992, 'Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines', Cancer Research, vol. 52, no. 5, pp. 1144-1148.
He X, Ostrowski LE, Friedman HS, Wikstrand CJ, Bigner SH, Rasheed A et al. Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines. Cancer Research. 1992 Jan 1;52(5):1144-1148.
He, Xuanmin ; Ostrowski, Lawrence E. ; Friedman, Henry S. ; Wikstrand, Carol J. ; Bigner, Sandra H. ; Rasheed, Ahmed ; Batra, Surinder Kumar ; Signer, Darell D. / Expression of O6-Methylguanine-DNA Methyltransferase in Six Human Medulloblastoma Cell Lines. In: Cancer Research. 1992 ; Vol. 52, No. 5. pp. 1144-1148.
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