Expression of neuronal nitric oxide synthase in rabbit carotid body glomus cells regulates large-conductance Ca2+-activated potassium currents

Yulong Li, Hong Zheng, Yanfeng Ding, Harold D Schultz

Research output: Contribution to journalArticle

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Abstract

Our previous studies show that a decrease in endogenous nitric oxide (NO) is involved in the blunted outward K+ currents in carotid body (CB) glomus cells from chronic heart failure (CHF) rabbits. In the present study, we measured the effects of the neuronal nitric oxide synthase (nNOS) transgene on the K+ currents in CB glomus cells from pacing-induced CHF rabbits. Using single-cell real-time RT-PCR and immunofluorescent techniques, we found that nNOS mRNA and protein are expressed in the rabbit CB glomus cells and CHF decreased the expression of nNOS mRNA and protein in CB glomus cells. After 3 days of an adenoviral nNOS (Ad.nNOS) gene transfection, the expression of nNOS protein was increased to the level found in sham CB glomus cells. In whole cell patch-clamp experiments, Ad.nNOS markedly reversed the attenuated K+ currents in CB glomus cells from CHF rabbits. The specific nNOS inhibitor (S-methyl-L-thiocitrulline [SMTC]) and large-conductance Ca2+- activated K+ (BK) channel blocker (iberiotoxin) fully abolished the effect of Ad.nNOS on the K+ currents in the CB glomus cells from CHF rabbits. However, neither CHF nor Ad.nNOS altered the protein expression of BK channel α-subunit. These results suggest that a decrease of NO induced by an attenuated nNOS activity lowers the activation of the BK channels but not the protein expression of the BK channel α-subunit in the CB glomus cells during CHF.

Original languageEnglish (US)
Pages (from-to)3027-3033
Number of pages7
JournalJournal of Neurophysiology
Volume103
Issue number6
DOIs
StatePublished - Jun 1 2010

Fingerprint

Carotid Body
Nitric Oxide Synthase Type I
Potassium
Rabbits
Large-Conductance Calcium-Activated Potassium Channels
Heart Failure
Proteins
Nitric Oxide
Calcium-Activated Potassium Channels
Messenger RNA
Transgenes
Transfection
Real-Time Polymerase Chain Reaction
Gene Expression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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Expression of neuronal nitric oxide synthase in rabbit carotid body glomus cells regulates large-conductance Ca2+-activated potassium currents. / Li, Yulong; Zheng, Hong; Ding, Yanfeng; Schultz, Harold D.

In: Journal of Neurophysiology, Vol. 103, No. 6, 01.06.2010, p. 3027-3033.

Research output: Contribution to journalArticle

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