Expression of mRNA electroporated into plant and animal cells

J. Cellis, M. Fromm, V. Walbot

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A general method to introduce RNA molecules into plant protoplasts and animal cells is described. This technique utilizes the ability of electric pulses of high field strength to form pores in biomembranes. RNA molecules containing the coding region for the bacterial enzyme chloramphenicol acetyltransferase (CAT) were used as a model system. The presence of CAT activity as a result of the in vivo translation of the introduced RNA is entirely dependent on the presence of a 5′ cap and greatly increased by the presence of a poly A tail at the 3′ end. The introduction of RNA into eukaiyotic cells has broad applicability both as an assay for the uptake of nucleic acids into cells independent of transcriptional activity and as a tool to study eukaryotic mRNA translation.

Original languageEnglish (US)
Pages (from-to)5823-5831
Number of pages9
JournalNucleic acids research
Volume15
Issue number14
DOIs
StatePublished - Jul 24 1987

Fingerprint

Plant Cells
RNA
Messenger RNA
Animals
Cells
Chloramphenicol O-Acetyltransferase
Cell
Molecules
Protoplast
Protoplasts
Tail
Nucleic acids
Enzymes
Protein Biosynthesis
Coding
Nucleic Acids
Assays
Dependent
Model
System model

ASJC Scopus subject areas

  • Genetics

Cite this

Expression of mRNA electroporated into plant and animal cells. / Cellis, J.; Fromm, M.; Walbot, V.

In: Nucleic acids research, Vol. 15, No. 14, 24.07.1987, p. 5823-5831.

Research output: Contribution to journalArticle

Cellis, J. ; Fromm, M. ; Walbot, V. / Expression of mRNA electroporated into plant and animal cells. In: Nucleic acids research. 1987 ; Vol. 15, No. 14. pp. 5823-5831.
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