Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers

Anshu Aggarwal, William J. Hunter, Himanshu Aggarwal, Edibaldo D. Silva, Mary S. Davey, Richard F. Murphy, Devendra K. Agrawal

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The POK family of proteins plays an important role in not only embryonic development and cell differentiation, but also in oncogenesis. Leukemia/lymphoma-related factor (LRF) belongs to the POK family of transcriptional repressors and is also known as POK erythroid myeloid ontogenic factor (POKEMON), which binds to short transcripts of HIV-1 (FBI-1) and TTF-1 interacting peptide (TIP21). Its oncogenic role is known only in lymphoma, non-small cell lung carcinoma, and malignant gliomas. The functional expression of LRF in human breast carcinoma has not yet been confirmed. The aim of this study was to investigate and compare the expression of LRF in human breast cancer tissues and other human tumors. The expression of LRF mRNA transcripts and protein was observed in twenty human benign and malignant breast biopsy tissues. Expression of LRF was observed in several formalin-fixed tissues by immunohistochemistry and immunofluorescence. All malignant breast tissues expressed mRNA transcripts and protein for LRF. However, 40% and 15% benign breast biopsy tissues expressed LRF mRNA transcripts and protein, respectively. The overall expression of LRF mRNA transcripts and total protein was significantly more in malignant breast tissues than the benign breast tissues. LRF expression was also observed in the nuclei of human colon, renal, lung, hepatocellular carcinomas and thymoma tumor cells. In general, a significantly higher expression of LRF was seen in malignant tissues than in the corresponding benign or normal tissue. Further studies are warranted to determine the malignant role of LRF in human breast carcinoma.

Original languageEnglish (US)
Pages (from-to)140-148
Number of pages9
JournalExperimental and Molecular Pathology
Volume89
Issue number2
DOIs
Publication statusPublished - Oct 1 2010

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Keywords

  • Breast cancer
  • LRF
  • POK
  • POKEMON
  • ZBTB7

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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