Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin

Rakesh K Singh, M. L. Varney, C. D. Bucana, S. L. Johansson

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

It was recently demonstrated that interleukin-8 (IL-8) is produced by cultured melanoma cells and acts as an essential autocrine growth factor. Earlier studies from our laboratory demonstrated a direct correlation between IL-8 expression in human variant cell lines and their metastatic potential in nude mice. In the present study we examined the expression of IL-8 in human malignant melanomas using immunohistochemistry to correlate IL-8 levels with disease stage. None of the radial growth phase (RGP) tumours (melanoma in situ) expressed IL-8. In contrast, 50% of the vertical growth phase (VGP) tumours (invasive melanoma), which have a high risk of metastasis, showed IL- 8 immunoreactivity. Further, all the metastatic lesions analysed showed intense staining for IL-8; the levels were higher than those observed in the primary skin tumours. In summary, the data suggest an association between the expression of IL-8 and the metastatic phenotype.

Original languageEnglish (US)
Pages (from-to)383-387
Number of pages5
JournalMelanoma Research
Volume9
Issue number4
StatePublished - Sep 6 1999

Fingerprint

Interleukin-8
Melanoma
Skin
Neoplasms
Growth
Nude Mice
Cultured Cells
Intercellular Signaling Peptides and Proteins
Immunohistochemistry
Staining and Labeling
Neoplasm Metastasis
Phenotype
Cell Line

Keywords

  • Interleukin-8
  • Melanoma
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Dermatology
  • Cancer Research

Cite this

Singh, R. K., Varney, M. L., Bucana, C. D., & Johansson, S. L. (1999). Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin. Melanoma Research, 9(4), 383-387.

Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin. / Singh, Rakesh K; Varney, M. L.; Bucana, C. D.; Johansson, S. L.

In: Melanoma Research, Vol. 9, No. 4, 06.09.1999, p. 383-387.

Research output: Contribution to journalArticle

Singh, RK, Varney, ML, Bucana, CD & Johansson, SL 1999, 'Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin', Melanoma Research, vol. 9, no. 4, pp. 383-387.
Singh, Rakesh K ; Varney, M. L. ; Bucana, C. D. ; Johansson, S. L. / Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin. In: Melanoma Research. 1999 ; Vol. 9, No. 4. pp. 383-387.
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