Expression of interleukin-8 in human metastatic endometrial carcinoma cells and its regulation by inflammatory cytokines

K. K. Berry, M. L. Varney, Bhavana J Dave, C. D. Bucana, I. J. Fidler, Rakesh K Singh

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In the present study, we analyzed the expression of a multifunctional cytokine, interleukin-8 (IL-8), in metastatic endometrial carcinoma cells. Our data demonstrate that human serous papillary endometrial adenocarcinoma (SPEC) and human endometrial adenocarcinoma (HEC) cells expressed steady-state IL-8-specific mRNA transcript and secreted IL-8 protein. The levels of IL-8 mRNA in SPEC-2 cells established from stage IV serous papillary adenocarcinoma were three-fold higher as compared to endometrial adenocarcinoma cells, HEC-1 A, established from stage IA endometrial cancer. Further, we observed higher levels of IL-8 mRNA and protein expression in the metastatic variants of SPEC-2 and HEC-1A cells as compared to the parent cell lines, demonstrating that IL-8 expression was associated with metastatic potential. Further, the treatment of endometrial carcinoma cells with inflammatory cytokines, IL-1β and tumor necrosis factor-α (TNF-α), demonstrated that IL-1β and TNF-α induced IL-8 expression in endometrial cancer cells. IL-1β was a more potent inducer of IL-8 expression than TNF-α in our studies. These data demonstrate that constitutive and induced IL-8 expression in endometrial carcinoma cells might be an important regulatory mechanism of tumor growth and metastasis.

Original languageEnglish (US)
Pages (from-to)54-60
Number of pages7
JournalInternational Journal of Gynecological Cancer
Volume11
Issue number1
DOIs
Publication statusPublished - Mar 26 2001

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Keywords

  • Cytokine
  • Interleukin-1
  • Interleukin-8
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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