Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

Rakesh K. Singh, Mordechai Gutman, Robert Radinsky, Corazon D. Bucana, Isaiah J. Fidler

Research output: Contribution to journalArticle

328 Citations (Scopus)

Abstract

We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

Original languageEnglish (US)
Pages (from-to)3242-3247
Number of pages6
JournalCancer Research
Volume54
Issue number12
StatePublished - Jun 15 1994

Fingerprint

Interleukin-8
Nude Mice
Melanoma
Messenger RNA
Neoplasm Metastasis
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Proteins
Enzyme-Linked Immunosorbent Assay
Cell Line
Antibodies

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Singh, R. K., Gutman, M., Radinsky, R., Bucana, C. D., & Fidler, I. J. (1994). Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice. Cancer Research, 54(12), 3242-3247.

Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice. / Singh, Rakesh K.; Gutman, Mordechai; Radinsky, Robert; Bucana, Corazon D.; Fidler, Isaiah J.

In: Cancer Research, Vol. 54, No. 12, 15.06.1994, p. 3242-3247.

Research output: Contribution to journalArticle

Singh, RK, Gutman, M, Radinsky, R, Bucana, CD & Fidler, IJ 1994, 'Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice', Cancer Research, vol. 54, no. 12, pp. 3242-3247.
Singh, Rakesh K. ; Gutman, Mordechai ; Radinsky, Robert ; Bucana, Corazon D. ; Fidler, Isaiah J. / Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice. In: Cancer Research. 1994 ; Vol. 54, No. 12. pp. 3242-3247.
@article{df1e4939412f42bd8912a35c736ba79c,
title = "Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice",
abstract = "We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.",
author = "Singh, {Rakesh K.} and Mordechai Gutman and Robert Radinsky and Bucana, {Corazon D.} and Fidler, {Isaiah J.}",
year = "1994",
month = "6",
day = "15",
language = "English (US)",
volume = "54",
pages = "3242--3247",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "12",

}

TY - JOUR

T1 - Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

AU - Singh, Rakesh K.

AU - Gutman, Mordechai

AU - Radinsky, Robert

AU - Bucana, Corazon D.

AU - Fidler, Isaiah J.

PY - 1994/6/15

Y1 - 1994/6/15

N2 - We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

AB - We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

UR - http://www.scopus.com/inward/record.url?scp=0028364780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028364780&partnerID=8YFLogxK

M3 - Article

C2 - 8205546

AN - SCOPUS:0028364780

VL - 54

SP - 3242

EP - 3247

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 12

ER -