Expression of human prostatic acid phosphatase gene is regulated by upstream negative and positive elements

Stanislav Zelivianski, Christopher Larson, James Seberger, Rodney Taylor, Ming-Fong Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. To understand the regulation of expression of the PAcP gene, we studied the cis-regulatory elements of its promoter. A DNA fragment from -2899 to +87 base pairs (bp) of PAcP gene was fused to the chloramphenicol acetyltransferase (CAT) reporter gene and introduced into PC-3 and LNCaP human prostate cancer cells. The expression of the CAT gene driven by the PAcP promoter was assessed in transient expression assays. Sequential 5' deletions of the promoter were constructed and analyzed to reveal the positive and the negative regulatory elements that are involved in regulating the transcription of the PAcP gene. Our data showed that the proximal sequence -1305/+87 bp directs a high level of the CAT activity in both cell lines. Deletion of the region from -1305 to -779 resulted in approximately a 10- and three-fold decrease of the PAcP promoter activity in PC-3 and LNCaP cells, respectively. Interestingly, an inverse correlation of the CAT activity with the cell growth was observed when the reporter gene was driven by the -1305/+87 fragment, but not by the -779/+87 fragment. Two regions of transcriptional suppression were identified and located in positions from -2899 to -2583, and from -2583 to -1305 bp. Furthermore, the activity of the core promoter region from -779 to +87 bp can be activated by a SV-40 enhancer. The results, thus, clearly demonstrate the presence of positive and negative cis-elements in the promoter region of the PAcP gene. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1491
Issue number1-3
DOIs
StatePublished - Apr 25 2000

Fingerprint

Genes
Chloramphenicol O-Acetyltransferase
Base Pairing
Reporter Genes
Genetic Promoter Regions
Cells
Differentiation Antigens
Cell growth
Transcription
Prostate-Specific Antigen
prostatic acid phosphatase
Assays
Prostatic Neoplasms
Epithelium
Cell Line
DNA
Growth

Keywords

  • Cell growth regulation
  • Promoter
  • Prostatic acid phosphatase
  • Transcription regulation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Expression of human prostatic acid phosphatase gene is regulated by upstream negative and positive elements. / Zelivianski, Stanislav; Larson, Christopher; Seberger, James; Taylor, Rodney; Lin, Ming-Fong.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1491, No. 1-3, 25.04.2000, p. 123-132.

Research output: Contribution to journalArticle

Zelivianski, Stanislav ; Larson, Christopher ; Seberger, James ; Taylor, Rodney ; Lin, Ming-Fong. / Expression of human prostatic acid phosphatase gene is regulated by upstream negative and positive elements. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2000 ; Vol. 1491, No. 1-3. pp. 123-132.
@article{f037154b1fe744a4bde9f858c770b0e1,
title = "Expression of human prostatic acid phosphatase gene is regulated by upstream negative and positive elements",
abstract = "Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. To understand the regulation of expression of the PAcP gene, we studied the cis-regulatory elements of its promoter. A DNA fragment from -2899 to +87 base pairs (bp) of PAcP gene was fused to the chloramphenicol acetyltransferase (CAT) reporter gene and introduced into PC-3 and LNCaP human prostate cancer cells. The expression of the CAT gene driven by the PAcP promoter was assessed in transient expression assays. Sequential 5' deletions of the promoter were constructed and analyzed to reveal the positive and the negative regulatory elements that are involved in regulating the transcription of the PAcP gene. Our data showed that the proximal sequence -1305/+87 bp directs a high level of the CAT activity in both cell lines. Deletion of the region from -1305 to -779 resulted in approximately a 10- and three-fold decrease of the PAcP promoter activity in PC-3 and LNCaP cells, respectively. Interestingly, an inverse correlation of the CAT activity with the cell growth was observed when the reporter gene was driven by the -1305/+87 fragment, but not by the -779/+87 fragment. Two regions of transcriptional suppression were identified and located in positions from -2899 to -2583, and from -2583 to -1305 bp. Furthermore, the activity of the core promoter region from -779 to +87 bp can be activated by a SV-40 enhancer. The results, thus, clearly demonstrate the presence of positive and negative cis-elements in the promoter region of the PAcP gene. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Cell growth regulation, Promoter, Prostatic acid phosphatase, Transcription regulation",
author = "Stanislav Zelivianski and Christopher Larson and James Seberger and Rodney Taylor and Ming-Fong Lin",
year = "2000",
month = "4",
day = "25",
doi = "10.1016/S0167-4781(00)00037-3",
language = "English (US)",
volume = "1491",
pages = "123--132",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "1-3",

}

TY - JOUR

T1 - Expression of human prostatic acid phosphatase gene is regulated by upstream negative and positive elements

AU - Zelivianski, Stanislav

AU - Larson, Christopher

AU - Seberger, James

AU - Taylor, Rodney

AU - Lin, Ming-Fong

PY - 2000/4/25

Y1 - 2000/4/25

N2 - Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. To understand the regulation of expression of the PAcP gene, we studied the cis-regulatory elements of its promoter. A DNA fragment from -2899 to +87 base pairs (bp) of PAcP gene was fused to the chloramphenicol acetyltransferase (CAT) reporter gene and introduced into PC-3 and LNCaP human prostate cancer cells. The expression of the CAT gene driven by the PAcP promoter was assessed in transient expression assays. Sequential 5' deletions of the promoter were constructed and analyzed to reveal the positive and the negative regulatory elements that are involved in regulating the transcription of the PAcP gene. Our data showed that the proximal sequence -1305/+87 bp directs a high level of the CAT activity in both cell lines. Deletion of the region from -1305 to -779 resulted in approximately a 10- and three-fold decrease of the PAcP promoter activity in PC-3 and LNCaP cells, respectively. Interestingly, an inverse correlation of the CAT activity with the cell growth was observed when the reporter gene was driven by the -1305/+87 fragment, but not by the -779/+87 fragment. Two regions of transcriptional suppression were identified and located in positions from -2899 to -2583, and from -2583 to -1305 bp. Furthermore, the activity of the core promoter region from -779 to +87 bp can be activated by a SV-40 enhancer. The results, thus, clearly demonstrate the presence of positive and negative cis-elements in the promoter region of the PAcP gene. Copyright (C) 2000 Elsevier Science B.V.

AB - Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. To understand the regulation of expression of the PAcP gene, we studied the cis-regulatory elements of its promoter. A DNA fragment from -2899 to +87 base pairs (bp) of PAcP gene was fused to the chloramphenicol acetyltransferase (CAT) reporter gene and introduced into PC-3 and LNCaP human prostate cancer cells. The expression of the CAT gene driven by the PAcP promoter was assessed in transient expression assays. Sequential 5' deletions of the promoter were constructed and analyzed to reveal the positive and the negative regulatory elements that are involved in regulating the transcription of the PAcP gene. Our data showed that the proximal sequence -1305/+87 bp directs a high level of the CAT activity in both cell lines. Deletion of the region from -1305 to -779 resulted in approximately a 10- and three-fold decrease of the PAcP promoter activity in PC-3 and LNCaP cells, respectively. Interestingly, an inverse correlation of the CAT activity with the cell growth was observed when the reporter gene was driven by the -1305/+87 fragment, but not by the -779/+87 fragment. Two regions of transcriptional suppression were identified and located in positions from -2899 to -2583, and from -2583 to -1305 bp. Furthermore, the activity of the core promoter region from -779 to +87 bp can be activated by a SV-40 enhancer. The results, thus, clearly demonstrate the presence of positive and negative cis-elements in the promoter region of the PAcP gene. Copyright (C) 2000 Elsevier Science B.V.

KW - Cell growth regulation

KW - Promoter

KW - Prostatic acid phosphatase

KW - Transcription regulation

UR - http://www.scopus.com/inward/record.url?scp=0034712768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034712768&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(00)00037-3

DO - 10.1016/S0167-4781(00)00037-3

M3 - Article

C2 - 10760575

AN - SCOPUS:0034712768

VL - 1491

SP - 123

EP - 132

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 1-3

ER -