Expression of FSH receptor in the hamster ovary during perinatal development

Prabuddha Chakraborty, Shyamal K Roy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

FSH plays an important role in ovarian follicular development, and it functions via the G-protein coupled FSH receptor. The objectives of the present study were to determine if full-length FSHR mRNA and corresponding protein were expressed in fetal through postnatal hamster ovaries to explain the FSH-induced primordial follicle formation, and if FSH or estrogen (E) would affect the expression. A full-length and two alternately spliced FSHR transcripts were expressed from E14 through P20. The level of the full-length FSHR mRNA increased markedly through P7 before stabilizing at a lower level with the formation and activation of primordial follicles. A predicted 87 kDa FSHR protein band was detected in fetal through P4 ovaries, but additional bands appeared as ovary developed. FSHR immunosignal was present in undifferentiated somatic cells and oocytes in early postnatal ovaries, but was granulosa cells specific after follicles formed. Both eCG and E significantly up-regulated full-length FSHR mRNA levels. Therefore, FSHR is expressed in the hamster ovary from the fetal life to account for FSH-induced primordial follicle formation and cAMP production. Further, FSH or E regulates the receptor expression.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume400
DOIs
StatePublished - Jan 5 2015

Fingerprint

FSH Receptors
Cricetinae
Ovary
Messenger RNA
GTP-Binding Proteins
Estrogens
Proteins
Chemical activation
Granulosa Cells
G-Protein-Coupled Receptors
Oocytes

Keywords

  • FSH
  • FSH-receptor
  • Ovary
  • Primordial follicle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Expression of FSH receptor in the hamster ovary during perinatal development. / Chakraborty, Prabuddha; Roy, Shyamal K.

In: Molecular and Cellular Endocrinology, Vol. 400, 05.01.2015, p. 41-47.

Research output: Contribution to journalArticle

@article{c7f7bf955a044a51853894e9fe310e40,
title = "Expression of FSH receptor in the hamster ovary during perinatal development",
abstract = "FSH plays an important role in ovarian follicular development, and it functions via the G-protein coupled FSH receptor. The objectives of the present study were to determine if full-length FSHR mRNA and corresponding protein were expressed in fetal through postnatal hamster ovaries to explain the FSH-induced primordial follicle formation, and if FSH or estrogen (E) would affect the expression. A full-length and two alternately spliced FSHR transcripts were expressed from E14 through P20. The level of the full-length FSHR mRNA increased markedly through P7 before stabilizing at a lower level with the formation and activation of primordial follicles. A predicted 87 kDa FSHR protein band was detected in fetal through P4 ovaries, but additional bands appeared as ovary developed. FSHR immunosignal was present in undifferentiated somatic cells and oocytes in early postnatal ovaries, but was granulosa cells specific after follicles formed. Both eCG and E significantly up-regulated full-length FSHR mRNA levels. Therefore, FSHR is expressed in the hamster ovary from the fetal life to account for FSH-induced primordial follicle formation and cAMP production. Further, FSH or E regulates the receptor expression.",
keywords = "FSH, FSH-receptor, Ovary, Primordial follicle",
author = "Prabuddha Chakraborty and Roy, {Shyamal K}",
year = "2015",
month = "1",
day = "5",
doi = "10.1016/j.mce.2014.11.014",
language = "English (US)",
volume = "400",
pages = "41--47",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Expression of FSH receptor in the hamster ovary during perinatal development

AU - Chakraborty, Prabuddha

AU - Roy, Shyamal K

PY - 2015/1/5

Y1 - 2015/1/5

N2 - FSH plays an important role in ovarian follicular development, and it functions via the G-protein coupled FSH receptor. The objectives of the present study were to determine if full-length FSHR mRNA and corresponding protein were expressed in fetal through postnatal hamster ovaries to explain the FSH-induced primordial follicle formation, and if FSH or estrogen (E) would affect the expression. A full-length and two alternately spliced FSHR transcripts were expressed from E14 through P20. The level of the full-length FSHR mRNA increased markedly through P7 before stabilizing at a lower level with the formation and activation of primordial follicles. A predicted 87 kDa FSHR protein band was detected in fetal through P4 ovaries, but additional bands appeared as ovary developed. FSHR immunosignal was present in undifferentiated somatic cells and oocytes in early postnatal ovaries, but was granulosa cells specific after follicles formed. Both eCG and E significantly up-regulated full-length FSHR mRNA levels. Therefore, FSHR is expressed in the hamster ovary from the fetal life to account for FSH-induced primordial follicle formation and cAMP production. Further, FSH or E regulates the receptor expression.

AB - FSH plays an important role in ovarian follicular development, and it functions via the G-protein coupled FSH receptor. The objectives of the present study were to determine if full-length FSHR mRNA and corresponding protein were expressed in fetal through postnatal hamster ovaries to explain the FSH-induced primordial follicle formation, and if FSH or estrogen (E) would affect the expression. A full-length and two alternately spliced FSHR transcripts were expressed from E14 through P20. The level of the full-length FSHR mRNA increased markedly through P7 before stabilizing at a lower level with the formation and activation of primordial follicles. A predicted 87 kDa FSHR protein band was detected in fetal through P4 ovaries, but additional bands appeared as ovary developed. FSHR immunosignal was present in undifferentiated somatic cells and oocytes in early postnatal ovaries, but was granulosa cells specific after follicles formed. Both eCG and E significantly up-regulated full-length FSHR mRNA levels. Therefore, FSHR is expressed in the hamster ovary from the fetal life to account for FSH-induced primordial follicle formation and cAMP production. Further, FSH or E regulates the receptor expression.

KW - FSH

KW - FSH-receptor

KW - Ovary

KW - Primordial follicle

UR - http://www.scopus.com/inward/record.url?scp=84914165730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914165730&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2014.11.014

DO - 10.1016/j.mce.2014.11.014

M3 - Article

C2 - 25462586

AN - SCOPUS:84914165730

VL - 400

SP - 41

EP - 47

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

ER -