Expression of dnaK and groESL operons during sporulation of Bacillus megaterium

Otakar Hlaváček, Jiří Adamec, Tomáš Vomastek, Lenka Babková, Miroslav Sedlák, Jiří Vohradský, Libuše Váchová, Jiří Chaloupka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increase in dnaK and groEL expression was determined at the same time. Synthesis of DnaK and GroEL encoding mRNAs during sporulation in non-shocked cells was low suggesting that this kind of cytodifferentiation was not accompanied by enhanced synthesis of these chaperones. Also the ability of sporulating cells to respond to a heat shock by stimulating their synthesis substantially decreased during the reversible and dropped to negligible values during the irreversible sporulation phase. Nevertheless, some dependence of the heat shock response on sporulation exists because sporulation suppression by mutation or by netropsin treatment further decreased the cells' capacity to respond to a heat shock. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalFEMS Microbiology Letters
Volume165
Issue number1
DOIs
StatePublished - Aug 1 1998

Fingerprint

Bacillus megaterium
Operon
Shock
Hot Temperature
Netropsin
Heat-Shock Response
Messenger RNA
Northern Blotting
Mutation
Genes
Proteins

Keywords

  • Bacillus megaterium
  • Gene expression
  • Heat shock protein
  • Sporulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Hlaváček, O., Adamec, J., Vomastek, T., Babková, L., Sedlák, M., Vohradský, J., ... Chaloupka, J. (1998). Expression of dnaK and groESL operons during sporulation of Bacillus megaterium. FEMS Microbiology Letters, 165(1), 181-186. https://doi.org/10.1016/S0378-1097(98)00274-2

Expression of dnaK and groESL operons during sporulation of Bacillus megaterium. / Hlaváček, Otakar; Adamec, Jiří; Vomastek, Tomáš; Babková, Lenka; Sedlák, Miroslav; Vohradský, Jiří; Váchová, Libuše; Chaloupka, Jiří.

In: FEMS Microbiology Letters, Vol. 165, No. 1, 01.08.1998, p. 181-186.

Research output: Contribution to journalArticle

Hlaváček, O, Adamec, J, Vomastek, T, Babková, L, Sedlák, M, Vohradský, J, Váchová, L & Chaloupka, J 1998, 'Expression of dnaK and groESL operons during sporulation of Bacillus megaterium', FEMS Microbiology Letters, vol. 165, no. 1, pp. 181-186. https://doi.org/10.1016/S0378-1097(98)00274-2
Hlaváček, Otakar ; Adamec, Jiří ; Vomastek, Tomáš ; Babková, Lenka ; Sedlák, Miroslav ; Vohradský, Jiří ; Váchová, Libuše ; Chaloupka, Jiří. / Expression of dnaK and groESL operons during sporulation of Bacillus megaterium. In: FEMS Microbiology Letters. 1998 ; Vol. 165, No. 1. pp. 181-186.
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