Expression of Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress

Saadia Bihmidine, Mingxia Cao, Ming Kang, Tala Awada, James L. Van Etten, David D. Dunigan, Tom E. Clemente

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Main conclusions: A Chlorovirus aquaglyceroporin expressed in tobacco is localized to the plastid and plasma membranes. Transgenic events display improved response to water deficit. Necrosis in adult stage plants is observed. Aquaglyceroporins are a subclass of the water channel aquaporin proteins (AQPs) that transport glycerol along with other small molecules transcellular in addition to water. In the studies communicated herein, we analyzed the expression of the aquaglyceroporin gene designated, aqpv1, from Chlorovirus MT325, in tobacco (Nicotiana tabacum), along with phenotypic changes induced by aqpv1 expression in planta. Interestingly, aqpv1 expression under control of either a constitutive or a root-preferred promoter, triggered local lesion formation in older leaves, which progressed significantly after induction of flowering. Fusion of aqpv1 with GFP suggests that the protein localized to the plasmalemma, and potentially with plastid and endoplasmic reticulum membranes. Physiological characterizations of transgenic plants during juvenile stage growth were monitored for potential mitigation to water dry-down (i.e., drought) and recovery. Phenotypic analyses on drought mimic/recovery of juvenile transgenic plants that expressed a functional aqpv1 transgene had higher photosynthetic rates, stomatal conductance, and water use efficiency, along with maximum carboxylation and electron transport rates when compared to control plants. These physiological attributes permitted the juvenile aqpv1 transgenic plants to perform better under drought-mimicked conditions and hastened recovery following re-watering. This drought mitigation effect is linked to the ability of the transgenic plants to maintain cell turgor.

Original languageEnglish (US)
Pages (from-to)209-221
Number of pages13
JournalPlanta
Volume240
Issue number1
DOIs
StatePublished - Jun 2014

Fingerprint

Chlorovirus
Aquaglyceroporins
Genetically Modified Plants
Droughts
Tobacco
transgenic plants
tobacco
water stress
drought
Aquaporins
Plastids
Water
plastids
plasma membrane
water
lesions (plant)
aquaporins
protein transport
carboxylation
Protein Transport

Keywords

  • Agrobacterium
  • Aquaglyceroporins
  • Aquaporins
  • Chlorovirus MT325
  • Local lesions
  • Water use efficiency

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Expression of Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress. / Bihmidine, Saadia; Cao, Mingxia; Kang, Ming; Awada, Tala; Van Etten, James L.; Dunigan, David D.; Clemente, Tom E.

In: Planta, Vol. 240, No. 1, 06.2014, p. 209-221.

Research output: Contribution to journalArticle

Bihmidine, Saadia ; Cao, Mingxia ; Kang, Ming ; Awada, Tala ; Van Etten, James L. ; Dunigan, David D. ; Clemente, Tom E. / Expression of Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress. In: Planta. 2014 ; Vol. 240, No. 1. pp. 209-221.
@article{393cf8aaff734adcbf547c7c1d88357e,
title = "Expression of Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress",
abstract = "Main conclusions: A Chlorovirus aquaglyceroporin expressed in tobacco is localized to the plastid and plasma membranes. Transgenic events display improved response to water deficit. Necrosis in adult stage plants is observed. Aquaglyceroporins are a subclass of the water channel aquaporin proteins (AQPs) that transport glycerol along with other small molecules transcellular in addition to water. In the studies communicated herein, we analyzed the expression of the aquaglyceroporin gene designated, aqpv1, from Chlorovirus MT325, in tobacco (Nicotiana tabacum), along with phenotypic changes induced by aqpv1 expression in planta. Interestingly, aqpv1 expression under control of either a constitutive or a root-preferred promoter, triggered local lesion formation in older leaves, which progressed significantly after induction of flowering. Fusion of aqpv1 with GFP suggests that the protein localized to the plasmalemma, and potentially with plastid and endoplasmic reticulum membranes. Physiological characterizations of transgenic plants during juvenile stage growth were monitored for potential mitigation to water dry-down (i.e., drought) and recovery. Phenotypic analyses on drought mimic/recovery of juvenile transgenic plants that expressed a functional aqpv1 transgene had higher photosynthetic rates, stomatal conductance, and water use efficiency, along with maximum carboxylation and electron transport rates when compared to control plants. These physiological attributes permitted the juvenile aqpv1 transgenic plants to perform better under drought-mimicked conditions and hastened recovery following re-watering. This drought mitigation effect is linked to the ability of the transgenic plants to maintain cell turgor.",
keywords = "Agrobacterium, Aquaglyceroporins, Aquaporins, Chlorovirus MT325, Local lesions, Water use efficiency",
author = "Saadia Bihmidine and Mingxia Cao and Ming Kang and Tala Awada and {Van Etten}, {James L.} and Dunigan, {David D.} and Clemente, {Tom E.}",
year = "2014",
month = "6",
doi = "10.1007/s00425-014-2075-5",
language = "English (US)",
volume = "240",
pages = "209--221",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Expression of Chlorovirus MT325 aquaglyceroporin (aqpv1) in tobacco and its role in mitigating drought stress

AU - Bihmidine, Saadia

AU - Cao, Mingxia

AU - Kang, Ming

AU - Awada, Tala

AU - Van Etten, James L.

AU - Dunigan, David D.

AU - Clemente, Tom E.

PY - 2014/6

Y1 - 2014/6

N2 - Main conclusions: A Chlorovirus aquaglyceroporin expressed in tobacco is localized to the plastid and plasma membranes. Transgenic events display improved response to water deficit. Necrosis in adult stage plants is observed. Aquaglyceroporins are a subclass of the water channel aquaporin proteins (AQPs) that transport glycerol along with other small molecules transcellular in addition to water. In the studies communicated herein, we analyzed the expression of the aquaglyceroporin gene designated, aqpv1, from Chlorovirus MT325, in tobacco (Nicotiana tabacum), along with phenotypic changes induced by aqpv1 expression in planta. Interestingly, aqpv1 expression under control of either a constitutive or a root-preferred promoter, triggered local lesion formation in older leaves, which progressed significantly after induction of flowering. Fusion of aqpv1 with GFP suggests that the protein localized to the plasmalemma, and potentially with plastid and endoplasmic reticulum membranes. Physiological characterizations of transgenic plants during juvenile stage growth were monitored for potential mitigation to water dry-down (i.e., drought) and recovery. Phenotypic analyses on drought mimic/recovery of juvenile transgenic plants that expressed a functional aqpv1 transgene had higher photosynthetic rates, stomatal conductance, and water use efficiency, along with maximum carboxylation and electron transport rates when compared to control plants. These physiological attributes permitted the juvenile aqpv1 transgenic plants to perform better under drought-mimicked conditions and hastened recovery following re-watering. This drought mitigation effect is linked to the ability of the transgenic plants to maintain cell turgor.

AB - Main conclusions: A Chlorovirus aquaglyceroporin expressed in tobacco is localized to the plastid and plasma membranes. Transgenic events display improved response to water deficit. Necrosis in adult stage plants is observed. Aquaglyceroporins are a subclass of the water channel aquaporin proteins (AQPs) that transport glycerol along with other small molecules transcellular in addition to water. In the studies communicated herein, we analyzed the expression of the aquaglyceroporin gene designated, aqpv1, from Chlorovirus MT325, in tobacco (Nicotiana tabacum), along with phenotypic changes induced by aqpv1 expression in planta. Interestingly, aqpv1 expression under control of either a constitutive or a root-preferred promoter, triggered local lesion formation in older leaves, which progressed significantly after induction of flowering. Fusion of aqpv1 with GFP suggests that the protein localized to the plasmalemma, and potentially with plastid and endoplasmic reticulum membranes. Physiological characterizations of transgenic plants during juvenile stage growth were monitored for potential mitigation to water dry-down (i.e., drought) and recovery. Phenotypic analyses on drought mimic/recovery of juvenile transgenic plants that expressed a functional aqpv1 transgene had higher photosynthetic rates, stomatal conductance, and water use efficiency, along with maximum carboxylation and electron transport rates when compared to control plants. These physiological attributes permitted the juvenile aqpv1 transgenic plants to perform better under drought-mimicked conditions and hastened recovery following re-watering. This drought mitigation effect is linked to the ability of the transgenic plants to maintain cell turgor.

KW - Agrobacterium

KW - Aquaglyceroporins

KW - Aquaporins

KW - Chlorovirus MT325

KW - Local lesions

KW - Water use efficiency

UR - http://www.scopus.com/inward/record.url?scp=84902983571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902983571&partnerID=8YFLogxK

U2 - 10.1007/s00425-014-2075-5

DO - 10.1007/s00425-014-2075-5

M3 - Article

C2 - 24797278

AN - SCOPUS:84902983571

VL - 240

SP - 209

EP - 221

JO - Planta

JF - Planta

SN - 0032-0935

IS - 1

ER -