Expression of CD27 and ischemia/reperfusion-induced expression of its ligand Siva in rat kidneys

Babu J Padanilam, Andrew J.P. Lewington, Marc R. Hammerman

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background. Studies identifying genes that are differentially expressed following induction of acute ischemic injury have been useful in delineating the pathophysiology of acute renal failure. Methods. A differential cDNA library screening technique was used to identify genes that are differentially expressed in rat kidney following induction of acute ischemic renal injury. Results. Levels of mRNA with a high homology to that coding for Siva, a human proapoptotic protein, were increased approximately 4.5-fold in kidneys obtained from rats within 12 hours following ischemia, compared to kidneys from sham-operated rats. A partial cDNA sequence for the rat protein (rat Siva) was determined that overlaps 92% of the human open reading frame. The cDNA sequence predicts a protein 177 amino acids in length with 76% homology to human Siva. Levels of rat Siva in kidneys were elevated at one, five and seven days post-ischemia. However, levels in kidneys obtained two days post-ischemia were not different from those in kidneys from sham- operated controls. In situ hybridization demonstrated that rat Siva mRNA was expressed in cells lining damaged sections in the S3 segment of the proximal tubule at 12 hours and one day post-ischemia. At five and seven days, Siva mRNA was located in epithelial cells of regenerating tubules including in papillary proliferations. TdT-mediated dUTP-biotin nick end-labeling (TUNEL)- positive cells colocalized with cells containing Siva mRNA. CD27, the receptor for Siva was localized by immunohistochemistry to sloughed cells in the lumens of damaged S3 segments at 12 hours post-ischemia and to cells within papillary proliferations at five days post-injury. Conclusions. Siva that is produced within the kidney could be a mediator of apoptosis post- ischemia via an interaction with CD27.

Original languageEnglish (US)
Pages (from-to)1967-1975
Number of pages9
JournalKidney International
Volume54
Issue number6
DOIs
StatePublished - Jan 1 1998

Fingerprint

Reperfusion
Ischemia
Ligands
Kidney
Messenger RNA
Acute Kidney Injury
Complementary DNA
Wounds and Injuries
Biotin
Gene Library
Open Reading Frames
Genes
In Situ Hybridization
Epithelial Cells
Immunohistochemistry
Apoptosis
Amino Acids
Proteins

Keywords

  • Apoptosis
  • Death domain
  • Fas
  • Ischemia/reperfusion injury
  • Regeneration
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Nephrology

Cite this

Expression of CD27 and ischemia/reperfusion-induced expression of its ligand Siva in rat kidneys. / Padanilam, Babu J; Lewington, Andrew J.P.; Hammerman, Marc R.

In: Kidney International, Vol. 54, No. 6, 01.01.1998, p. 1967-1975.

Research output: Contribution to journalArticle

Padanilam, Babu J ; Lewington, Andrew J.P. ; Hammerman, Marc R. / Expression of CD27 and ischemia/reperfusion-induced expression of its ligand Siva in rat kidneys. In: Kidney International. 1998 ; Vol. 54, No. 6. pp. 1967-1975.
@article{f8e97621dd5047acbc8a70c5e4033e89,
title = "Expression of CD27 and ischemia/reperfusion-induced expression of its ligand Siva in rat kidneys",
abstract = "Background. Studies identifying genes that are differentially expressed following induction of acute ischemic injury have been useful in delineating the pathophysiology of acute renal failure. Methods. A differential cDNA library screening technique was used to identify genes that are differentially expressed in rat kidney following induction of acute ischemic renal injury. Results. Levels of mRNA with a high homology to that coding for Siva, a human proapoptotic protein, were increased approximately 4.5-fold in kidneys obtained from rats within 12 hours following ischemia, compared to kidneys from sham-operated rats. A partial cDNA sequence for the rat protein (rat Siva) was determined that overlaps 92{\%} of the human open reading frame. The cDNA sequence predicts a protein 177 amino acids in length with 76{\%} homology to human Siva. Levels of rat Siva in kidneys were elevated at one, five and seven days post-ischemia. However, levels in kidneys obtained two days post-ischemia were not different from those in kidneys from sham- operated controls. In situ hybridization demonstrated that rat Siva mRNA was expressed in cells lining damaged sections in the S3 segment of the proximal tubule at 12 hours and one day post-ischemia. At five and seven days, Siva mRNA was located in epithelial cells of regenerating tubules including in papillary proliferations. TdT-mediated dUTP-biotin nick end-labeling (TUNEL)- positive cells colocalized with cells containing Siva mRNA. CD27, the receptor for Siva was localized by immunohistochemistry to sloughed cells in the lumens of damaged S3 segments at 12 hours post-ischemia and to cells within papillary proliferations at five days post-injury. Conclusions. Siva that is produced within the kidney could be a mediator of apoptosis post- ischemia via an interaction with CD27.",
keywords = "Apoptosis, Death domain, Fas, Ischemia/reperfusion injury, Regeneration, Tumor necrosis factor",
author = "Padanilam, {Babu J} and Lewington, {Andrew J.P.} and Hammerman, {Marc R.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1046/j.1523-1755.1998.00197.x",
language = "English (US)",
volume = "54",
pages = "1967--1975",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Expression of CD27 and ischemia/reperfusion-induced expression of its ligand Siva in rat kidneys

AU - Padanilam, Babu J

AU - Lewington, Andrew J.P.

AU - Hammerman, Marc R.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Background. Studies identifying genes that are differentially expressed following induction of acute ischemic injury have been useful in delineating the pathophysiology of acute renal failure. Methods. A differential cDNA library screening technique was used to identify genes that are differentially expressed in rat kidney following induction of acute ischemic renal injury. Results. Levels of mRNA with a high homology to that coding for Siva, a human proapoptotic protein, were increased approximately 4.5-fold in kidneys obtained from rats within 12 hours following ischemia, compared to kidneys from sham-operated rats. A partial cDNA sequence for the rat protein (rat Siva) was determined that overlaps 92% of the human open reading frame. The cDNA sequence predicts a protein 177 amino acids in length with 76% homology to human Siva. Levels of rat Siva in kidneys were elevated at one, five and seven days post-ischemia. However, levels in kidneys obtained two days post-ischemia were not different from those in kidneys from sham- operated controls. In situ hybridization demonstrated that rat Siva mRNA was expressed in cells lining damaged sections in the S3 segment of the proximal tubule at 12 hours and one day post-ischemia. At five and seven days, Siva mRNA was located in epithelial cells of regenerating tubules including in papillary proliferations. TdT-mediated dUTP-biotin nick end-labeling (TUNEL)- positive cells colocalized with cells containing Siva mRNA. CD27, the receptor for Siva was localized by immunohistochemistry to sloughed cells in the lumens of damaged S3 segments at 12 hours post-ischemia and to cells within papillary proliferations at five days post-injury. Conclusions. Siva that is produced within the kidney could be a mediator of apoptosis post- ischemia via an interaction with CD27.

AB - Background. Studies identifying genes that are differentially expressed following induction of acute ischemic injury have been useful in delineating the pathophysiology of acute renal failure. Methods. A differential cDNA library screening technique was used to identify genes that are differentially expressed in rat kidney following induction of acute ischemic renal injury. Results. Levels of mRNA with a high homology to that coding for Siva, a human proapoptotic protein, were increased approximately 4.5-fold in kidneys obtained from rats within 12 hours following ischemia, compared to kidneys from sham-operated rats. A partial cDNA sequence for the rat protein (rat Siva) was determined that overlaps 92% of the human open reading frame. The cDNA sequence predicts a protein 177 amino acids in length with 76% homology to human Siva. Levels of rat Siva in kidneys were elevated at one, five and seven days post-ischemia. However, levels in kidneys obtained two days post-ischemia were not different from those in kidneys from sham- operated controls. In situ hybridization demonstrated that rat Siva mRNA was expressed in cells lining damaged sections in the S3 segment of the proximal tubule at 12 hours and one day post-ischemia. At five and seven days, Siva mRNA was located in epithelial cells of regenerating tubules including in papillary proliferations. TdT-mediated dUTP-biotin nick end-labeling (TUNEL)- positive cells colocalized with cells containing Siva mRNA. CD27, the receptor for Siva was localized by immunohistochemistry to sloughed cells in the lumens of damaged S3 segments at 12 hours post-ischemia and to cells within papillary proliferations at five days post-injury. Conclusions. Siva that is produced within the kidney could be a mediator of apoptosis post- ischemia via an interaction with CD27.

KW - Apoptosis

KW - Death domain

KW - Fas

KW - Ischemia/reperfusion injury

KW - Regeneration

KW - Tumor necrosis factor

UR - http://www.scopus.com/inward/record.url?scp=0031756662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031756662&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.1998.00197.x

DO - 10.1046/j.1523-1755.1998.00197.x

M3 - Article

VL - 54

SP - 1967

EP - 1975

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 6

ER -