Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco

Edgar B. Cahoon, John Shanklin, John B. Ohlrogge

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Little is known about the metabolic origin of petroselinic acid (18:1Δ6cis), the principal fatty acid of the seed oil of most Umbelliferae, Araliaceae, and Garryaceae species. To examine the possibility that petroselinic acid is the product of an acyl-acyl carrier protein (ACP) desaturase, Western blots of coriander and other Umbelliferae seed extracts were probed with antibodies against the Δ9-stearoyl-ACP desaturase of avocado. In these extracts, proteins of 39 and 36 kDa were detected. Of these, only the 36-kDa peptide was specific to tissues which synthesize petroselinic acid. A cDNA encoding the 36-kDa peptide was isolated from a coriander endosperm cDNA library, placed under control of the cauliflower mosaic virus 35S promoter, and introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Expression of this cDNA in transgenic tobacco callus was accompanied by the accumulation of petroselinic acid and Δ4-hexadecenoic acid, both of which were absent from control callus. These results demonstrate the involvement of a 36-kDa putative acyl-ACP desaturase in the biosynthetic pathway of petroselinic acid and the ability to produce fatty acids of unusual structure in transgenic plants by the expression of the gene for this desaturase.

Original languageEnglish (US)
Pages (from-to)11184-11188
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number23
DOIs
StatePublished - Dec 1 1992

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Coriandrum
acyl-(acyl-carrier-protein)desaturase
Tobacco
Apiaceae
Bony Callus
Seeds
Fatty Acids
Araliaceae
Complementary DNA
Persea
Caulimovirus
Plant Genes
Agrobacterium tumefaciens
Endosperm
Peptides
Genetically Modified Plants
Biosynthetic Pathways
Gene Library
Oils
Western Blotting

Keywords

  • Fatty acid desaturation
  • Transgenic expression
  • Umbelliferae
  • Unsaturated fatty acid

ASJC Scopus subject areas

  • General

Cite this

Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. / Cahoon, Edgar B.; Shanklin, John; Ohlrogge, John B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 23, 01.12.1992, p. 11184-11188.

Research output: Contribution to journalArticle

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