Expression of β-defensins in gingival health and in periodontal disease

John Bissell, Sophie Joly, Georgia K. Johnson, Connie C. Organ, Deborah Dawson, Paul B. McCray, Janet M. Guthmiller

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

BACKGROUND: Human β-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease. METHODS: Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of β-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic. RESULTS: All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001). CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.

Original languageEnglish (US)
Pages (from-to)278-285
Number of pages8
JournalJournal of Oral Pathology and Medicine
Volume33
Issue number5
DOIs
StatePublished - May 1 2004

Fingerprint

Defensins
Periodontal Diseases
Messenger RNA
Health
Gingival Diseases
Mouth Mucosa
Gingiva
Infection
Salivary Glands
Reverse Transcription
RNA
Polymerase Chain Reaction
Peptides

Keywords

  • Antimicrobial peptides
  • Innate immunity
  • Periodontal disease/immunology
  • Polymerase chain reaction/methods
  • β-defensins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Expression of β-defensins in gingival health and in periodontal disease. / Bissell, John; Joly, Sophie; Johnson, Georgia K.; Organ, Connie C.; Dawson, Deborah; McCray, Paul B.; Guthmiller, Janet M.

In: Journal of Oral Pathology and Medicine, Vol. 33, No. 5, 01.05.2004, p. 278-285.

Research output: Contribution to journalArticle

Bissell, John ; Joly, Sophie ; Johnson, Georgia K. ; Organ, Connie C. ; Dawson, Deborah ; McCray, Paul B. ; Guthmiller, Janet M. / Expression of β-defensins in gingival health and in periodontal disease. In: Journal of Oral Pathology and Medicine. 2004 ; Vol. 33, No. 5. pp. 278-285.
@article{1c7c44f1ad6a414781fe3b5c66504c16,
title = "Expression of β-defensins in gingival health and in periodontal disease",
abstract = "BACKGROUND: Human β-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease. METHODS: Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of β-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic. RESULTS: All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001). CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.",
keywords = "Antimicrobial peptides, Innate immunity, Periodontal disease/immunology, Polymerase chain reaction/methods, β-defensins",
author = "John Bissell and Sophie Joly and Johnson, {Georgia K.} and Organ, {Connie C.} and Deborah Dawson and McCray, {Paul B.} and Guthmiller, {Janet M.}",
year = "2004",
month = "5",
day = "1",
doi = "10.1111/j.0904-2512.2004.00143.x",
language = "English (US)",
volume = "33",
pages = "278--285",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Expression of β-defensins in gingival health and in periodontal disease

AU - Bissell, John

AU - Joly, Sophie

AU - Johnson, Georgia K.

AU - Organ, Connie C.

AU - Dawson, Deborah

AU - McCray, Paul B.

AU - Guthmiller, Janet M.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - BACKGROUND: Human β-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease. METHODS: Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of β-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic. RESULTS: All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001). CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.

AB - BACKGROUND: Human β-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease. METHODS: Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of β-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic. RESULTS: All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001). CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.

KW - Antimicrobial peptides

KW - Innate immunity

KW - Periodontal disease/immunology

KW - Polymerase chain reaction/methods

KW - β-defensins

UR - http://www.scopus.com/inward/record.url?scp=2342589520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342589520&partnerID=8YFLogxK

U2 - 10.1111/j.0904-2512.2004.00143.x

DO - 10.1111/j.0904-2512.2004.00143.x

M3 - Article

C2 - 15078488

AN - SCOPUS:2342589520

VL - 33

SP - 278

EP - 285

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 5

ER -