Expression artifact with retroviral vectors based on pBMN

Yoshitaka Fukunaga, Robert A. Svoboda, Ronald L. Cerny, Keith R. Johnson, Margaret J. Wheelock

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalAnalytical Biochemistry
Volume395
Issue number1
DOIs
StatePublished - Dec 1 2009

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Keywords

  • LZRS
  • Retroviral vector
  • p120 Catenin
  • pBMN

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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