Expression artifact with retroviral vectors based on pBMN

Yoshitaka Fukunaga, Robert A. Svoboda, Ronald Cerny, Keith R Johnson, Margaret J. Wheelock

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalAnalytical Biochemistry
Volume395
Issue number1
DOIs
StatePublished - Dec 1 2009

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Artifacts
Molecular Weight
Molecular weight
Post Translational Protein Processing
Fusion reactions
Research Personnel
Proteins
delta catenin

Keywords

  • LZRS
  • Retroviral vector
  • p120 Catenin
  • pBMN

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Expression artifact with retroviral vectors based on pBMN. / Fukunaga, Yoshitaka; Svoboda, Robert A.; Cerny, Ronald; Johnson, Keith R; Wheelock, Margaret J.

In: Analytical Biochemistry, Vol. 395, No. 1, 01.12.2009, p. 49-53.

Research output: Contribution to journalArticle

Fukunaga, Yoshitaka ; Svoboda, Robert A. ; Cerny, Ronald ; Johnson, Keith R ; Wheelock, Margaret J. / Expression artifact with retroviral vectors based on pBMN. In: Analytical Biochemistry. 2009 ; Vol. 395, No. 1. pp. 49-53.
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