Abstract
This study evaluated the expression and regulation of β-defensins DEFB-104 and the recently identified DEFB-105-14 in gingival keratinocytes. Keratinocytes from healthy subjects were exposed to cytokines, Escherichia coli lipopolysaccharide or Candida species. Total RNA was extracted and defensin expression analyzed by reverse transcription-polymerase chain reaction. Three patterns of expression were seen: no expression, constitutive expression and inducible expression. Constitutive mRNA expression was evident for DEFB-104, 107, 109, 111, and 112. DEFB-108 and 114 were induced by interleukin (IL)-1β and Candida species. For DEFB-108 expression, synergism was observed when IL-1β was combined with tumor necrosis factor-α or interferon-γ. Downregulation of DEFB-109 occurred following treatment with Candida albicans. These findings suggest a role for multiple β-defensins in response to oral infection. Further investigation is needed to better understand their function, both in terms of antimicrobial activities and contributions to innate and acquired immunity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 111-117 |
| Number of pages | 7 |
| Journal | Oral Microbiology and Immunology |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1 2004 |
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Keywords
- Antimicrobial peptides
- Gene expression
- Gingival keratinocytes
- Innate immunity
- β-defensins
ASJC Scopus subject areas
- Microbiology
- Immunology
- Dentistry(all)
- Microbiology (medical)
Cite this
Expression and regulation of novel human β-defensins in gingival keratinocytes. / Premratanachai, P.; Joly, S.; Johnson, G. K.; McCray, P. B.; Jia, H. P.; Guthmiller, Janet M.
In: Oral Microbiology and Immunology, Vol. 19, No. 2, 01.04.2004, p. 111-117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and regulation of novel human β-defensins in gingival keratinocytes
AU - Premratanachai, P.
AU - Joly, S.
AU - Johnson, G. K.
AU - McCray, P. B.
AU - Jia, H. P.
AU - Guthmiller, Janet M.
PY - 2004/4/1
Y1 - 2004/4/1
N2 - This study evaluated the expression and regulation of β-defensins DEFB-104 and the recently identified DEFB-105-14 in gingival keratinocytes. Keratinocytes from healthy subjects were exposed to cytokines, Escherichia coli lipopolysaccharide or Candida species. Total RNA was extracted and defensin expression analyzed by reverse transcription-polymerase chain reaction. Three patterns of expression were seen: no expression, constitutive expression and inducible expression. Constitutive mRNA expression was evident for DEFB-104, 107, 109, 111, and 112. DEFB-108 and 114 were induced by interleukin (IL)-1β and Candida species. For DEFB-108 expression, synergism was observed when IL-1β was combined with tumor necrosis factor-α or interferon-γ. Downregulation of DEFB-109 occurred following treatment with Candida albicans. These findings suggest a role for multiple β-defensins in response to oral infection. Further investigation is needed to better understand their function, both in terms of antimicrobial activities and contributions to innate and acquired immunity.
AB - This study evaluated the expression and regulation of β-defensins DEFB-104 and the recently identified DEFB-105-14 in gingival keratinocytes. Keratinocytes from healthy subjects were exposed to cytokines, Escherichia coli lipopolysaccharide or Candida species. Total RNA was extracted and defensin expression analyzed by reverse transcription-polymerase chain reaction. Three patterns of expression were seen: no expression, constitutive expression and inducible expression. Constitutive mRNA expression was evident for DEFB-104, 107, 109, 111, and 112. DEFB-108 and 114 were induced by interleukin (IL)-1β and Candida species. For DEFB-108 expression, synergism was observed when IL-1β was combined with tumor necrosis factor-α or interferon-γ. Downregulation of DEFB-109 occurred following treatment with Candida albicans. These findings suggest a role for multiple β-defensins in response to oral infection. Further investigation is needed to better understand their function, both in terms of antimicrobial activities and contributions to innate and acquired immunity.
KW - Antimicrobial peptides
KW - Gene expression
KW - Gingival keratinocytes
KW - Innate immunity
KW - β-defensins
UR - http://www.scopus.com/inward/record.url?scp=1442338497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1442338497&partnerID=8YFLogxK
U2 - 10.1111/j.0902-0055.2002.00127.x
DO - 10.1111/j.0902-0055.2002.00127.x
M3 - Article
C2 - 14871351
AN - SCOPUS:1442338497
VL - 19
SP - 111
EP - 117
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
SN - 2041-1006
IS - 2
ER -