Exposure to UV light causes increased biotinylation of histones in Jurkat cells

Dorothea M. Peters, Jacob B. Griffin, J. Steven Stanley, Mary M. Beck, Janos Zempleni

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Biotin in breakdown products of biotinylated carboxylases serves as substrate for biotinylation of histones by biotinidase. Here we determined whether biotinylation of histones might play a role in repair of damaged DNA and in apoptosis. Jurkat cells were exposed to UV light to induce DNA damage. Abundance of thymine dimers increased about three times in response to UV exposure, consistent with DNA damage. Biotin-containing carboxylases were degraded in response to UV exposure, as judged by Western blot analysis and carboxylase activities. Mitochondrial integrity decreased in response to UV exposure (as judged by confocal microscopy), facilitating the release of breakdown products of carboxylases from mitochondria. Biotinylation of histones increased in response to UV exposure; biotinylation of histones did not occur specifically at sites of newly repaired DNA. UV exposure triggered apoptosis, as judged by caspase-3 activity and analysis by confocal microscopy. In summary, this study provided evidence that increased biotinylation of histones in DNA-damaged cells might either be a side product of carboxylase degradation or a step during apoptosis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number3 52-3
StatePublished - Sep 1 2002

Fingerprint

Biotinylation
Jurkat Cells
Ultraviolet Rays
Ultraviolet radiation
Histones
DNA
biotin carboxylase
Confocal microscopy
Apoptosis
Biotin
Confocal Microscopy
DNA Damage
Biotinidase
Pyrimidine Dimers
Mitochondria
Caspase 3
DNA Repair
Repair
Western Blotting
Degradation

Keywords

  • Apoptosis
  • Biotin
  • Carboxylases
  • DNA damage

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Exposure to UV light causes increased biotinylation of histones in Jurkat cells. / Peters, Dorothea M.; Griffin, Jacob B.; Steven Stanley, J.; Beck, Mary M.; Zempleni, Janos.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 3 52-3, 01.09.2002.

Research output: Contribution to journalArticle

Peters, Dorothea M. ; Griffin, Jacob B. ; Steven Stanley, J. ; Beck, Mary M. ; Zempleni, Janos. / Exposure to UV light causes increased biotinylation of histones in Jurkat cells. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 3 52-3.
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