Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila

Robert Farkaš, Michaela Kováčiková, Denisa Liszeková, Milan Beňo, Peter Daniš, Leonard Rabinow, Bruce A. Chase, Ivan Raška

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Members of the highly conserved LAMMER family of protein kinases have been described in all eukaryotes. LAMMER kinases possess markedly similar peptide motifs in their kinase catalytic subdomains that are responsible for phosphotransfer and substrate interaction, suggesting that family members serve similar functions in widely diverged species. This hypothesis is supported by their phosphorylation of SR and SR-related proteins in diverged species. Here we describe a 3-dimensional homology model of the catalytic domain of DOA, a representative LAMMER kinase, encoded by the Drosophila locus Darkener of apricot (Doa). Homology modeling of DOA based on a Sky1p template revealed a highly conserved structural framework within conserved core regions. These adopt typical kinase folding like that of other protein kinases. However, in contrast to Sky1p, some structural features, such as those in helix αC suggest that the DOA kinase is not a constitutively active enzyme but requires activation. This may occur by phosphorylation within an activation loop that forms a broad turn and in which interactions between the side chains occur across the loop. The fold of the activation loop is stabilized through interactions with residues in the C-terminal tail, which is not part of the conserved kinase core and is variable among protein kinases. Immediately following the activation loop in the segment between the β9 sheet and helix αF is a P + 1 loop. The electrostatic surface potential of the DOA substrate-binding groove is largely negative, as it is in other known SR protein kinases, suggesting that DOA substrates must be basic. All differences between D. melanogaster and other Drosophila species are single amino acid changes situated in regions outside of any α-helices or β-sheets, and after modeling these had absolutely no visible effect on protein structure. The absence of evolved amino acid changes among 12 Drosophila species that would cause at least predictable changes in DOA structure indicate that evolution has already selected evolved mutations for having minimal effect on kinase structure.

Original languageEnglish (US)
Pages (from-to)130-142
Number of pages13
JournalFly
Volume3
Issue number2
DOIs
StatePublished - Jan 1 2009

Fingerprint

protein kinases
Drosophila
phosphotransferases (kinases)
physicochemical properties
phosphorylation
Melanogaster
amino acids
apricots
protein structure
active sites
eukaryotic cells
tail
peptides
mutation
loci
enzymes
proteins

Keywords

  • DOA
  • Drosophila
  • Dual-specific protein kinases
  • Functional diversity
  • Molecular models
  • Protein structure

ASJC Scopus subject areas

  • Insect Science

Cite this

Farkaš, R., Kováčiková, M., Liszeková, D., Beňo, M., Daniš, P., Rabinow, L., ... Raška, I. (2009). Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila. Fly, 3(2), 130-142. https://doi.org/10.4161/fly.7831

Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila. / Farkaš, Robert; Kováčiková, Michaela; Liszeková, Denisa; Beňo, Milan; Daniš, Peter; Rabinow, Leonard; Chase, Bruce A.; Raška, Ivan.

In: Fly, Vol. 3, No. 2, 01.01.2009, p. 130-142.

Research output: Contribution to journalArticle

Farkaš, R, Kováčiková, M, Liszeková, D, Beňo, M, Daniš, P, Rabinow, L, Chase, BA & Raška, I 2009, 'Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila', Fly, vol. 3, no. 2, pp. 130-142. https://doi.org/10.4161/fly.7831
Farkaš R, Kováčiková M, Liszeková D, Beňo M, Daniš P, Rabinow L et al. Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila. Fly. 2009 Jan 1;3(2):130-142. https://doi.org/10.4161/fly.7831
Farkaš, Robert ; Kováčiková, Michaela ; Liszeková, Denisa ; Beňo, Milan ; Daniš, Peter ; Rabinow, Leonard ; Chase, Bruce A. ; Raška, Ivan. / Exploring some of the physico-chemical properties of the lamer protein kinase doa of Drosophila. In: Fly. 2009 ; Vol. 3, No. 2. pp. 130-142.
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