Experimental Retinal Detachment

Vii. Intravenous Horseradish Peroxidase Diffusion Across the Blood-Retinal Barrier

Carol B Toris, Jonathan E. Pederson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Intravenous horseradish peroxidase (HRP) was administered to cynomolgus monkeys to study chorioretinal diffusion in eyes with retinal detachment. In control eyes, HRP was contained within the choriocapillaris and did not penetrate beyond Bruch's membrane. In addition, HRP was confined within blood vessels of the sensory retina. In detached eyes, HRP diffused out of the choriocapillaris and through the intercellular spaces of the retinal pigment epithelium (RPE), where it was blocked by zonulae occludentes. Occasionally HRP was found within pinocytotic vesicles of the RPE. It was also confined within retinal blood vessels by their endothelial cells. It is concluded that the blood-retinal barrier remains intact to HRP following long-term rhegmatogenous retinal detachment. However, a slight alteration exists at the choriocapillaris in detached eyes.

Original languageEnglish (US)
Pages (from-to)752-756
Number of pages5
JournalArchives of Ophthalmology
Volume102
Issue number5
DOIs
StatePublished - Jan 1 1984

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Blood-Retinal Barrier
Retinal Detachment
Horseradish Peroxidase
Retinal Pigment Epithelium
Bruch Membrane
Retinal Vessels
Macaca fascicularis
Extracellular Space
Blood Vessels
Retina
Endothelial Cells

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Experimental Retinal Detachment : Vii. Intravenous Horseradish Peroxidase Diffusion Across the Blood-Retinal Barrier. / Toris, Carol B; Pederson, Jonathan E.

In: Archives of Ophthalmology, Vol. 102, No. 5, 01.01.1984, p. 752-756.

Research output: Contribution to journalArticle

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